1个试剂盒足以用于150个显微镜玻片 (18 x 18 mm),其中含有:封闭缓冲液 (1 X),22.5 mL
HRP-偶联链霉素亲和素 (1 X),22.5 mL
Alexa Fluor 酪胺试剂
过氧化氢(稳定的 3% 溶液
常见问题解答 (FAQ)
With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?
To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.
How are SuperBoost tyramide kits different from the original TSA labeling kits?
The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.
Immunofluorescent staining is central to nearly all cell-based research, yet only a few fluorescent signal amplification approaches for cell staining exist, each with distinct limitations. Here, the authors present a novel, fluorescent polymerization-based amplification (FPBA) method that is shown to enable similar signal intensities as the highly sensitive, enzyme-based tyramide ... More
FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts.
Authors:Smith ER, Tan SJ, Holt SG, Hewitson TD,
Journal:Sci Rep
PubMed ID:28611350
In kidney disease, higher circulating levels of the mineral-regulating hormone fibroblast growth factor (FGF)-23 are predictive of disease progression but direct pathogenic effects on the kidney are unknown. We sought evidence of local renal synthesis in response to unilateral ureteric obstruction in the mouse, and pro-fibrotic actions of FGF23 on ... More