TBE 缓冲液(Tris-硼酸盐-EDTA)(10X)
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TBE 缓冲液(Tris-硼酸盐-EDTA)(10X)
Thermo Scientific™

TBE 缓冲液(Tris-硼酸盐-EDTA)(10X)

Thermo Scientific 10X TBE 缓冲液(Tris-硼酸盐-EDTA)是 DNA 聚丙烯酰胺凝胶电泳和 RNA了解更多信息
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货号数量
B521 L
货号 B52
价格(CNY)
467.00
飞享价
Ends: 31-Dec-2025
666.00
共减 199.00 (30%)
Each
添加至购物车
数量:
1 L
请求批量或定制报价
价格(CNY)
467.00
飞享价
Ends: 31-Dec-2025
666.00
共减 199.00 (30%)
Each
添加至购物车
Thermo Scientific 10X TBE 缓冲液(Tris-硼酸盐-EDTA)是 DNA 聚丙烯酰胺凝胶电泳和 RNA 聚丙烯酰胺凝胶电泳中应用较广泛的缓冲液。TBE 与非变性凝胶或变性(7 M 尿素)凝胶配合使用。它也常规用于 DNA 自动化测序凝胶。TBE 也可用于琼脂糖凝胶,但不建议用于回收核酸的制备性凝胶。因为 TBE 缓冲液中的硼酸盐是多种酶的强抑制剂,所以在观察 DNA 样品中酶的应用时,推荐使用 TAE 缓冲液(Tris 乙酸酯-EDTA 缓冲液,10X 粉,sc-296647)。

应用

• 核酸琼脂糖凝胶电泳和核酸聚丙烯酰胺凝胶电泳
• 用作跑胶缓冲液和凝胶制备缓冲液
• 经由 0.22 μm 的膜进行过滤
• 推荐用于小于 1500 bp 的 RNA 和 DNA 片段的电泳


双链线性核酸分子在 TBE 缓冲液中的移动速度比在 TAE 缓冲液中慢 10% 左右。
仅供科研使用。不可用于诊断程序。
规格
产品类型TBE 缓冲液
数量1 L
最大浓度10X
Unit SizeEach

常见问题解答 (FAQ)

For the purpose of recovering and solubilizing nucleic acids in alcohol, can TAE running buffer be used instead of TBE running buffer when running a pre-cast TBE gel?

A TAE running buffer used in conjunction with a TBE gel would create high current and may lead to gel hydrolysis. Also, the acetate ion in TAE migrates faster than the borate ion in TBE buffer, and can create fuzzy bands. It is possible to try soaking the TBE gel (run in TBE buffer) in the TAE buffer following the run and then eluting from that. Since nucleic acids diffuse out of the gel relatively slowly, this may work.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.