MultiShot™ StripWell DH5α T1R 感受态细胞

MultiShot StripWell DH5α T1R 化学感受态大肠杆菌细胞是克隆感受态细胞，包装在一个含 12 联排 8 孔的支架中，以提高中等通量细菌转化的生产率。MultiShot了解更多信息

货号	数量
C449601	1 块板

货号 C449601

价格（CNY)

7,195.00

添加至购物车

价格（CNY)

7,195.00

添加至购物车

MultiShot StripWell DH5α T1^R 化学感受态大肠杆菌细胞是克隆感受态细胞，包装在一个含 12 联排 8 孔的支架中，以提高中等通量细菌转化的生产率。MultiShot StripWell DH5α T1^R 感受态大肠杆菌来源于常用的 DH5α 菌株，这是较常用的实验室克隆菌株之一。所有 T1^R 菌株的基因型均缺失 tonA (fhuA)，对 T1 和 T5 噬菌体具有抗性。T1 噬菌体迅速传播并使大肠杆菌宿主裂解，通常用于克隆和文库构建。抗性基因型增加了安全性，以保护宝贵的克隆和文库。这对于基因组和测序中心尤其重要，因为在这些中心，T1 噬菌体感染将是灾难性的。

可靠的 DH5α T1^R 克隆能力
DH5α 菌株是化学转换用标准实验室菌株，因为其适用于转化克隆未甲基化 DNA。DH5α T1^R 菌株可用于许多克隆应用，且提供的转化效率 >1 x 10⁸ cfu/µg 对照质粒 DNA。

MultiShot StripWell DH5α T1^R 感受态细胞的主要特点包括：
•hsdR 用于通过 PCR 扩增高效转化未甲基化 DNA
• lacZΔM15 用于蓝白斑筛选重组克隆
• 由于无需进行核酸内切酶 I 的非特异性酶切，endA1 用于在下游应用中获得更清洁的 DNA 制备物和更好的结果
• recA1 用于降低克隆 DNA 中的非特异性重组
• tonA (fhuA) 提供对 T1 和 T5 噬菌体的抗性

MultiShot StripWell 规格—灵活，无浪费
8个连接管的每个 StripWell 设计为与 One Shot 规格管具有相同的单管、单次使用特点。该规格可让您在同一管中完成至平板接种的转化方案的所有步骤，从而帮助您节省时间并防止污染。添加 DNA 后，MultiShot StripWell 感受态细胞可使用水浴器在 42°C 下全部在同一管中通过热激转化。

可切割 StripWell 管从而在单管中进行转化，避免浪费细胞等分试样并避免可能导致转化效率降低的冻融。

MultiShot StripWell 规格的主要特点包括：
•生产率提高—适用于中等通量实验
• 极佳的灵活性—从 StripWell 支架转换至少1个至多96支管
• 熟悉方便—无需等分，直接向细胞中加入 DNA，热激，回收至同一管中
• 让用户放心无忧—单次使用转化尽可能减少污染

基因型：F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96 relA1 tonA

查找您需要的细胞株和规格
我们提供化学感受态细胞和电转感受态细胞的许多其他大肠杆菌菌株和规格，以帮助满足您的特定转化需求。如果您需要其他高通量转化选项，请从我们的 MultiShot 格式化感受态细胞集合中选择，或通过电子邮箱联系我们自定义格式选项。

仅供科研使用。不可用于诊断程序。

耐抗生素细菌No

蓝色/白色筛查是

是否可克隆甲基化 DNA是

是否含 F' 附加体缺乏 F’附加体

高通量能力兼容高通量应用

制备无甲基化 DNA不适合制备未甲基化DNA

产品线MultiShot™, Stbl3™

产品类型感受态细胞

数量1 块板

减少克隆重组现象Yes (recA1)

运输条件干冰

T1 噬菌体 - 抗性 (tonA)是

产品规格MultiShot StripWell

种属大肠杆菌

Unit SizeEach

内容与储存

• MultiShot StripWell DH5α T1^R 感受态细胞：一个 96-stripwell 支架，50 µL/孔，在 –80°C 下储存
• pUC19 DNA (10 pg/µL)：1 小瓶，50 µL，在 –20°C 下储存
• S.O.C. 培养基：2 瓶，每瓶 15 mL，在 4°C 或室温下储存

常见问题解答 (FAQ)

Is it possible to perform a fast transformation protocol with the MultiShot competent cells?

There are situations where either because of time or process limitations, elimination of the heat shock and recovery time post-heat shock in the transformation protocol would be desirable. Testing was performed on many of our chemically competent cells in both the MultiShot StripWell and MultiShot FlexPlate format with a shortened transformation protocol. Use of a rapid transformation protocol (add DNA, no heat shock, no recovery period, plate on warm agar) yielded up to a log lower in transformation efficiency using the control DNA. This rapid transformation protocol can be performed with expectation of lower colony counts than our standard high efficiency transformation protocol and can only be performed with plasmids that use ampicillin as their antibiotic resistance. The rapid protocol is ideal when using a liquid handling system for high-throughput transformations with high cloning efficiency. Please see the product manual for full details on this rapid transformation protocol option.

Can I use a thermal cycler or heat block to heat shock the competent cells in the MultiShot FlexPlate?

The MultiShot FlexPlate is a PCR plate and can be placed in a thermal cycler or heat block set at 42 degrees C to transform the cells by the heat shock method. The duration of the heat shock performed in these devices has yielded similar transformation efficiencies as that seen with heat shock done in a water bath.

Which E. coli strains do you offer in the MultiShot StripWell format?

We offer the following E. coli strains in the MultiShot StripWell format: TOP10, DH5alpha T1R, Mach1 T1R, Stbl3, and BL21 Star (DE3) chemically competent cells.

Which E. coli strains do you offer in the MultiShot FlexPlate format?

We offer the following E. coli strains in the MultiShot FlexPlate format: TOP10, DH5alpha T1R, DH10B T1R, Mach1 T1R, OmniMAX2 T1R, and Stbl3 chemically competent cells.

What are the differences between the MultiShot StripWell, MultiShot FlexPlate, and MultiShot formats of E. coli cells?

The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.

The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.

The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.