MultiShot™ FlexPlate DH10B T1R 感受态细胞

MultiShot FlexPlate DH10B T1R 化学感受态大肠杆菌细胞是克隆感受态细胞，在 96 孔 PCR 板中预等分，以提高转化生产率。FlexPlate了解更多信息

货号	数量
C6481201	1 x 96 well plate (20 μl/well)

货号 C6481201

价格（CNY)

4,959.00

添加至购物车

1 x 96 well plate (20 μl/well)

价格（CNY)

4,959.00

添加至购物车

MultiShot FlexPlate DH10B T1^R 化学感受态大肠杆菌细胞是克隆感受态细胞，在 96 孔 PCR 板中预等分，以提高转化生产率。FlexPlate 规格可分成 8 孔段的任意组合，以实现转化通量的灵活性。

所有 T1^R 菌株的基因型均缺失 tonA (fhuA)，其对 T1 和 T5 噬菌体具有抗性。T1 噬菌体迅速传播并使大肠杆菌宿主裂解，通常用于克隆和文库构建。抗性基因型增加了安全性，以保护宝贵的克隆和文库。这对于基因组和测序中心尤其重要，因为在这些中心，T1 噬菌体感染将是灾难性的。

多种 DH10B T1^R 克隆能力
MultiShot FlexPlate DH10B T1^R 感受态大肠杆菌细胞可用于多种克隆应用，因为它们对连接酶和连接缓冲液的效应有抗性，且提供的转化效率 >1 x 10⁸ cfu/µg 对照质粒 DNA。该细胞还适用于克隆含有甲基胞嘧啶和甲基腺嘌呤的 DNA（即基因组 DNA），并且适用于克隆大质粒和 BAC。

MultiShot FlexPlate DH10B T1^R 感受态细胞的主要特点包括：
•mcrA、mcrBC、mrr 和 hsdRMS 限制系统的消除，以构建更具代表性的基因组构建体
•对 T1 和 T5 噬菌体具有抗性的 tonA (fhuA) 基因型
•endA1 用于增加质粒得率和数量
•recA1 用于降低克隆 DNA 中的非特异性重组
•lacZΔM15 用于重组克隆的蓝/白斑筛选

MultiShot FlexWell 规格—提高了转化生产率
FlexPlate 是一种 96 孔 PCR 板，可用于自动化液体处理系统或分成 8 孔段的任意组合，以实现转化通量的灵活性。可使用多通道移液管或液体处理系统快速将 DNA 添加至 MultiShot FlexPlate 感受态细胞。添加 DNA 后，细胞可使用水浴、加热块或热循环仪通过热激高效地转化。

MultiShot FlexPlate 规格的主要特点包括：
•提高通量—与自动化液体处理平台兼容
• 灵活—以 8 孔增量转化至整个 96 孔板
• 便利—感受态细胞预先等分至 96 孔板，可即用于转化

基因型：F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 lacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK rpsL nupG λ- tonA

查找您需要的细胞株和规格
我们提供化学感受态细胞和电转感受态细胞的许多其他大肠杆菌菌株和规格，以帮助满足您的特定转化需求。如果您需要其他高通量转化选项，请从我们的 MultiShot 格式化感受态细胞集合中选择，或通过电子邮箱联系我们自定义格式选项。

仅供科研使用。不可用于诊断程序。

耐抗生素细菌No

蓝色/白色筛查是

是否可克隆甲基化 DNA是

是否含 F' 附加体缺乏 F’附加体

高通量能力兼容高通量应用

质粒可用于 > 100 kb 质粒

制备无甲基化 DNA不适合制备未甲基化DNA

产品线MultiShot™, Stbl3™

产品类型感受态细胞

数量1 x 96 well plate (20 μl/well)

减少克隆重组现象Yes (recA1)

运输条件干冰

T1 噬菌体 - 抗性 (tonA)是

产品规格96 孔板

种属大肠杆菌

Unit SizeEach

内容与储存

• MultiShot FlexPlate DH10B T1^R 感受态细胞：一个 96 孔板，20 µL/孔，在 –80°C 下储存
• pUC19 DNA (10 pg/µL)：1 瓶，50 µL，在 –20°C 下储存
• S.O.C. 培养基：2 瓶，每瓶 10 mL，在 4°C 或室温下储存

常见问题解答 (FAQ)

Is it possible to perform a fast transformation protocol with the MultiShot competent cells?

There are situations where either because of time or process limitations, elimination of the heat shock and recovery time post-heat shock in the transformation protocol would be desirable. Testing was performed on many of our chemically competent cells in both the MultiShot StripWell and MultiShot FlexPlate format with a shortened transformation protocol. Use of a rapid transformation protocol (add DNA, no heat shock, no recovery period, plate on warm agar) yielded up to a log lower in transformation efficiency using the control DNA. This rapid transformation protocol can be performed with expectation of lower colony counts than our standard high efficiency transformation protocol and can only be performed with plasmids that use ampicillin as their antibiotic resistance. The rapid protocol is ideal when using a liquid handling system for high-throughput transformations with high cloning efficiency. Please see the product manual for full details on this rapid transformation protocol option.

Can I use a thermal cycler or heat block to heat shock the competent cells in the MultiShot FlexPlate?

The MultiShot FlexPlate is a PCR plate and can be placed in a thermal cycler or heat block set at 42 degrees C to transform the cells by the heat shock method. The duration of the heat shock performed in these devices has yielded similar transformation efficiencies as that seen with heat shock done in a water bath.

Which E. coli strains do you offer in the MultiShot StripWell format?

We offer the following E. coli strains in the MultiShot StripWell format: TOP10, DH5alpha T1R, Mach1 T1R, Stbl3, and BL21 Star (DE3) chemically competent cells.

Which E. coli strains do you offer in the MultiShot FlexPlate format?

We offer the following E. coli strains in the MultiShot FlexPlate format: TOP10, DH5alpha T1R, DH10B T1R, Mach1 T1R, OmniMAX2 T1R, and Stbl3 chemically competent cells.

What are the differences between the MultiShot StripWell, MultiShot FlexPlate, and MultiShot formats of E. coli cells?

The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.

The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.

The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.