Campylobacter Blood-Free Selective Agar Base (Dehydrated)

Isolate Campylobacter jejuni, Campylobacter coli and Campylobacter laridis using Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) when used with CCDA Selective Supplement SR0155. Campylobacter Blood-Free Selective Agar Base is based on the original formulation described by Bolton et.al¹ which was developed to replace blood with charcoal, ferrous sulphate and sodium pyruvate. For improved selectivity cephazolin in the formulation was replaced by cefoperazone as a selective agent².

Campylobacter are Gram-negative, spirally shaped microaerophilic organisms which may be present in raw milk, untreated water, improperly handled food and undercooked meats, poultry and shellfish. Human consumption of these organisms can result in a range of clinical illnesses from transient asymptomatic colonisation to severe dysentery. The symptoms of Campylobacter enteritis include diarrhoea, stomach pain, nausea, fever, headache and muscle pain.

Use Campylobacter Blood-Free Selective Agar Base for isolation of Campylobacter species.

• Selective: due to the presence of the selective agents like cefoperazone and amphotericin B the medium is selective for Campylobacter
• Suitable for isolation of Campylobacter spp from non-clinical samples following enrichment in Exeter broth³.
• Recommended by the U.K. Ministry of Agriculture, Fisheries and Food (MAFF) as a validated method for isolation of Campylobacter from foods.⁴

The colonial morphology of campylobacters can be used as a guideline for identification to species level.Campylobacter jejuni strains produce grey, moist flat spreading colonies. Some strains may have a green hue or a dry appearance, with or without a metallic sheen.Campylobacter coli strains tend to be creamy-grey in colour, moist, slightly raised and often produce discrete colonies.

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