ELISA 缓冲液试剂盒
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ELISA 缓冲液试剂盒
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ELISA 缓冲液试剂盒

ELISA 缓冲液试剂盒包含使用 Invitrogen 抗体对或运行 ELISA 所需的所有基础缓冲液。本试剂盒包含的材料足够用于 10 个 ELISA了解更多信息
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货号数量
CNB001110 plates
货号 CNB0011
价格(CNY)
5,545.00
飞享价
Ends: 31-Dec-2025
7,380.00
共减 1,835.00 (25%)
Each
添加至购物车
数量:
10 plates
价格(CNY)
5,545.00
飞享价
Ends: 31-Dec-2025
7,380.00
共减 1,835.00 (25%)
Each
添加至购物车
ELISA 缓冲液试剂盒包含使用 Invitrogen 抗体对或运行 ELISA 所需的所有基础缓冲液。本试剂盒包含的材料足够用于 10 个 ELISA 板。

试剂盒组成:
• 包被缓冲液 A(10 mM 磷酸盐缓冲液,pH 7.4)
• 包被缓冲液 B(50 mM 磷酸盐缓冲液,pH 9.4)
• 检测缓冲液(封闭缓冲液)(5X)
• 洗涤缓冲液 (25X)
• 稳定 TMB
• 终止液

查看所有可用的适用于 ELISA 应用的缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
适用于(应用)ELISA
形式浓缩液
运输条件湿冰
数量10 plates
Unit SizeEach
内容与储存
包被缓冲液 A (100 mL):1 瓶
包被缓冲液 B (100 mL):1 瓶
检测缓冲液 (5x) (200 mL):1 瓶
洗涤缓冲液 (25x) (100 mL):3 瓶
稳定显色剂 (25 mL):4 瓶
终止液 (100 mL):1 瓶存储在 2°C 到 8°C。

常见问题解答 (FAQ)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.