ChargeSwitch Pro Filter Plasmid Mini 或 Midi试剂盒纯化质粒的常规产量是多少?
通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒,midi试剂盒从25 mL菌液可得到200µg质粒,而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。
我想分离口腔细胞中的基因组DNA,该如何操作?
我们推荐使用MagMAX DNA Multi-Sample Ultra 2.0试剂盒或ChargeSwitch 口腔细胞基因组DNA试剂盒(https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/buccal-dna-extraction.html),这款产品专用于从口腔细胞样本中分离高品质的基因组DNA。同时我们也提供标准化试剂盒(normalized kits),确保用户针对不同样品分离所得的DNA浓度进行标准化处理。
I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?
We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.