Impact of hapten presentation on antibody binding at lipid membrane interfaces.
Authors:Jung H, Yang T, Lasagna MD, Shi J, Reinhart GD, Cremer PS,
Journal:Biophys J
PubMed ID:18199665
We report the effects of ligand presentation on the binding of aqueous proteins to solid supported lipid bilayers. Specifically, we show that the equilibrium dissociation constant can be strongly affected by ligand lipophilicity and linker length/structure. The apparent equilibrium dissociation constants (K(D)) were compared for two model systems, biotin/anti-biotin and ... More