DiSC3(5)（3,3'-二丙基硫杂二羰花青,碘化物）
DiSC<sub>3</sub>(5)（3,3'-二丙基硫杂二羰花青,碘化物）
引用和文献 (222)
Invitrogen™

DiSC3(5)（3,3'-二丙基硫杂二羰花青,碘化物）

电位探针 DiSC3(5) 是一种碳氰酸酯，带有一个短 (C3) 烷基尾部。该阳离子染料在超极化膜上积聚，并转运至脂质双分子层中。了解更多信息
Have Questions?
货号数量
D306100 mg
货号 D306
价格（CNY)
6,520.00
Each
添加至购物车
数量:
100 mg
价格（CNY)
6,520.00
Each
添加至购物车
电位探针 DiSC3(5) 是一种碳氰酸酯，带有一个短 (C3) 烷基尾部。该阳离子染料在超极化膜上积聚，并转运至脂质双分子层中。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量100 mg
运输条件室温
子细胞定位细胞膜&脂质
颜色红色
适用于（设备）荧光显微镜
产品类型DiSC3(5)
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。 这是什么原因所致？

由于这些染料插入脂质膜，任何对膜的破坏都将导致染料流失。这包括用Triton X-100之类的去垢剂或是甲醇之类的有机溶剂通透。通透是胞内抗体标记所必需的，但它会导致染料流失。相反，CFDA SE之类活性染料能够共价连接到细胞组分，因此可以在固定和通透之后更好的保留。

亲脂性示踪剂跨膜转运需要多久？FAST亲脂性染料能快多少？

转运过程相当缓慢：在活体组织中大约6 mm/天，在固定组织中更慢。亲脂性羰花青示踪剂从开始给样处扩散到神经元末端，可能需要数天甚至数周的时间。FAST DIO和DiI类似物（具有不饱和烷基尾）可以提高50％左右的转运速度。

我应该使用哪种亲脂性示踪剂（DiO、DiI、DiD等）？

请选择与您现有的激发光源和发射滤光片组/通道兼容的染料。固体、糊和晶体形式的染料可直接施加到组织中的神经元。如需标记培养的细胞或进行显微注射，可采用溶液或固态形式的亲脂性染料。

我想标记两种细胞群体然后进行细胞融合实验，哪种试剂最适合来成像？

亲脂性花青染料非常适合此类测定，因为它们会掺入到细胞膜中，随着细胞融合之后共用细胞膜，染料也随之共享。例如，一个细胞群体可以用DiI（橙红色）进行标记，而另一细胞群可以用DIO（绿色）进行标记，当细胞融合时合并的颜色为黄色（使用双带通滤光片成像）。

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all DiSC3(5)（3,3'-二丙基硫杂二羰花青,碘化物） FAQs

引用和文献 (222)

引用和文献
Abstract
Quantitation of corneal endothelial potentials using a carbocyanine dye.
Authors:Graves C, Sachs G
Journal:Biochim Biophys Acta
PubMed ID:6977376
The carbocyanine dye, diS-C3-(5) was used to quantitate the plasma membrane potential of the bullfrog corneal endothelium. It was shown that valinomycin hyperpolarized the endothelial cell and that in the presence of the ionophore the membrane potential largely reflected the K+ equilibrium potential. Using calibration curves constructed by changing medium ... More
Visualization of the intracellular behavior of HIV in living cells.
Authors:McDonald D, Vodicka MA, Lucero G, Svitkina TM, Borisy GG, Emerman M, Hope TJ
Journal:J Cell Biol
PubMed ID:12417576
'To track the behavior of human immunodeficiency virus (HIV)-1 in the cytoplasm of infected cells, we have tagged virions by incorporation of HIV Vpr fused to the GFP. Observation of the GFP-labeled particles in living cells revealed that they moved in curvilinear paths in the cytoplasm and accumulated in the ... More
Measurement of membrane potential in Bacillus subtilis: a comparison of lipophilic cations, rubidium ion, and a cyanine dye as probes.
Authors:Zaritsky A, Kihara M, Macnab RM
Journal:J Membr Biol
PubMed ID:6796695
Monitoring the effect of an anti-cancer drug on RPMI 4788 cells by a membrane potential probe, dis-C3-(5).
Authors:Iwagaki H, Fuchimoto S, Shiiki S, Miyake M, Orita K
Journal:J Med
PubMed ID:2504864
Use of intracellular calcium and membrane potential fluorescent indicators in neuroendocrine cells.
Authors:Capponi AM, Lew PD, Schlegel W, Pozzan T
Journal:Methods Enzymol
PubMed ID:3713520
View all DiSC3(5)（3,3'-二丙基硫杂二羰花青,碘化物） citations