DiA；4-Di-16-ASP（4-（4-（双十六烷基氨基）苯乙烯基）-N-甲基吡啶碘化物）
DiA；4-Di-16-ASP（4-（4-（双十六烷基氨基）苯乙烯基）-<i>N</i>-甲基吡啶碘化物）
Invitrogen™

DiA；4-Di-16-ASP（4-（4-（双十六烷基氨基）苯乙烯基）-N-甲基吡啶碘化物）

The lipophilic aminostyryl probe DiA inserts into membranes with its two alkyl tails and its fluorophore oriented parallel to the了解更多信息
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货号数量
D3883
又称 D-3883
25 mg
货号 D3883
又称 D-3883
价格（CNY)
4,998.00
Each
添加至购物车
数量:
25 mg
价格（CNY)
4,998.00
Each
添加至购物车
The lipophilic aminostyryl probe DiA inserts into membranes with its two alkyl tails and its fluorophore oriented parallel to the phospholipid acyl chain. When this dialkylaminostyryl probe binds to membranes, it exhibits a strong fluorescence enhancement; its fluorescence in water is minimal.
仅供科研使用。不可用于诊断程序。
规格
颜色黄色
检测方法荧光
适用于（设备）荧光显微镜
数量25 mg
运输条件室温
产品类型DiA 4-Di-16-ASP
亚细胞定位细胞膜&脂质, Lipids
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

我想标记细胞膜，但可选的染料有多种，该如何选择？

如果进行活细胞成像，CellVue和CellMask 质膜染色剂染色最均匀，被细胞内吞的速度最慢。如果想固定和通透细胞（如进行抗体标记），这种产品并不是最佳选择。麦胚凝集素（WGA）偶联物也能标记活细胞，或者甲醛固定的细胞。它们可以在随后的去垢剂（如TritonX-100）通透处理后保存下来。但如果细胞已经进行通透处理，WGA也会标记内部结构。因此，如果细胞已通透，则只能使用靶向质膜蛋白的抗体。亲脂性花青染料（如DiI）会标记活细胞的所有膜结构，而不仅仅是细胞质膜。此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html）的内容可帮助您进行选择。

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.