FAST DiI™ 油；DiIΔ^9,12-C₁₈(3)，ClO₄ (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐)

<i>FAST</i> DiI™ 油；DiIΔ<sup>9,12</sup>-C<sub>18</sub>(3)，ClO<sub>4</sub> (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐)

引用和文献 (42)

Invitrogen™

FAST DiI™ 油；DiIΔ^9,12-C₁₈(3)，ClO₄ (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐)

FAST DiI 是 DiI (DiIC18(3)) 的一种不饱和类似物，据报告迁移速度加快 ∼50%。其在水中发弱荧光，但在掺入膜中后会发出极其明亮且光稳定的荧光。在脂质环境中，其具有超高的消光系数和激发态寿命 (∼1了解更多信息

货号	数量
D3899	5 mg

货号 D3899

价格（CNY)

4,232.00

Ends: 31-Dec-2025

5,736.00

共减 1,504.00 (26%)

添加至购物车

价格（CNY)

4,232.00

Ends: 31-Dec-2025

5,736.00

共减 1,504.00 (26%)

添加至购物车

FAST DiI 是 DiI (DiIC₁₈(3)) 的一种不饱和类似物，据报告迁移速度加快 ∼50%。其在水中发弱荧光，但在掺入膜中后会发出极其明亮且光稳定的荧光。在脂质环境中，其具有超高的消光系数和激发态寿命 (∼1 ns)。添加到细胞中后，这种染料就会在细胞膜内侧向扩散。

仅供科研使用。不可用于诊断程序。

检测方法荧光

适用于（设备）荧光显微镜

产品线FAST DiI

数量5 mg

运输条件室温

标签类型Fluorescent Dye

产品类型DiIΔ9 12-C18(3) ClO4

亚细胞定位细胞膜&脂质, Lipids

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。这是什么原因所致？

由于这些染料插入脂质膜，任何对膜的破坏都将导致染料流失。这包括用Triton X-100之类的去垢剂或是甲醇之类的有机溶剂通透。通透是胞内抗体标记所必需的，但它会导致染料流失。相反，CFDA SE之类活性染料能够共价连接到细胞组分，因此可以在固定和通透之后更好的保留。

亲脂性示踪剂跨膜转运需要多久？FAST亲脂性染料能快多少？

转运过程相当缓慢：在活体组织中大约6 mm/天，在固定组织中更慢。亲脂性羰花青示踪剂从开始给样处扩散到神经元末端，可能需要数天甚至数周的时间。FAST DIO和DiI类似物（具有不饱和烷基尾）可以提高50％左右的转运速度。

我应该使用哪种亲脂性示踪剂（DiO、DiI、DiD等）？

请选择与您现有的激发光源和发射滤光片组/通道兼容的染料。固体、糊和晶体形式的染料可直接施加到组织中的神经元。如需标记培养的细胞或进行显微注射，可采用溶液或固态形式的亲脂性染料。

我想标记两种细胞群体然后进行细胞融合实验，哪种试剂最适合来成像？

亲脂性花青染料非常适合此类测定，因为它们会掺入到细胞膜中，随着细胞融合之后共用细胞膜，染料也随之共享。例如，一个细胞群体可以用DiI（橙红色）进行标记，而另一细胞群可以用DIO（绿色）进行标记，当细胞融合时合并的颜色为黄色（使用双带通滤光片成像）。

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FAST DiI™ 油；DiIΔ^9,12-C₁₈(3)，ClO₄ (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐) FAQs

引用和文献 (42)

引用和文献

Abstract

Reticulated lipid probe fluorescence reveals MDCK cell apical membrane topography.

Authors:Colarusso P, Spring KR

Journal:Biophys J

PubMed ID:11806917

High spatial resolution confocal microscopy of young MDCK cells stained with the lipophilic probe 1,1'-dihexadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiIC(16)) revealed a reticulated fluorescence pattern on the apical membrane. DiIC(16) was delivered as crystals to live cells to minimize possible solvent perturbations of the membrane lipids. The ratio of the integrated fluorescence ... More

Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion.

Authors:Laughlin RC, McGugan GC, Powell RR, Welter BH, Temesvari LA

Journal:Infect Immun

PubMed ID:15322032

'Lipid rafts are highly ordered, cholesterol-rich, and detergent-resistant microdomains found in the plasma membrane of many eukaryotic cells. These domains play important roles in endocytosis, secretion, and adhesion in a variety of cell types. The parasitic protozoan Entamoeba histolytica, the causative agent of amoebic dysentery, was determined to have raft-like ... More

Growth-associated protein-43 is required for commissural axon guidance in the developing vertebrate nervous system.

Authors:Shen Y, Mani S, Donovan SL, Schwob JE, Meiri KF

Journal:J Neurosci

PubMed ID:11756507

'Growth-associated protein-43 (GAP-43) is a major growth cone protein whose phosphorylation by PKC in response to extracellular guidance cues can regulate F-actin behavior. Here we show that 100% of homozygote GAP-43 (-/-) mice failed to form the anterior commissure (AC), hippocampal commissure (HC), and corpus callosum (CC) in vivo. Instead, ... More

Use of lipophilic dyes in studies of axonal pathfinding in vivo.

Authors:Perrin FE, Stoeckli ET

Journal:Microsc Res Tech

PubMed ID:10620782

'Fluorescent lipophilic dyes are an ideal tool to study axonal pathfinding. Because these dyes do not require active axonal transport for their spreading, they can be used in fixed tissue. Here, we describe the method we have used to study the molecular mechanisms of commissural axon pathfinding in the embryonic ... More

Differential projections of ciliated and microvillous olfactory receptor cells in the catfish, Ictalurus punctatus.

Authors:Morita Y, Finger TE

Journal:J Comp Neurol

PubMed ID:9717708

'The primary olfactory projections of channel catfish Ictalurus punctatus have been examined with postmortem tracing by using either 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate or 1,1-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). Following DiI deposition into small areas in different parts of the posterior olfactory bulb, olfactory sensory neurons always were labeled throughout the olfactory epithelium. No obvious ... More

View all FAST DiI™ 油；DiIΔ^9,12-C₁₈(3)，ClO₄ (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐) citations

FAST DiI™ 油；DiIΔ9,12-C18(3)，ClO4 (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐)

常见问题解答 (FAQ)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。 这是什么原因所致？

亲脂性示踪剂跨膜转运需要多久？FAST亲脂性染料能快多少？

我应该使用哪种亲脂性示踪剂（DiO、DiI、DiD等）？

我想标记两种细胞群体然后进行细胞融合实验，哪种试剂最适合来成像？

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

引用和文献 (42)

FAST DiI™ 油；DiIΔ^9,12-C₁₈(3)，ClO₄ (1,1'-二亚油醇酯-3,3,3',3'-四甲基吲哚羰花青高氯酸盐)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。这是什么原因所致？