SP-DiIC₁₈(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁)

SP-DiIC<sub>18</sub>(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁)

引用和文献 (15)

Invitrogen™

SP-DiIC₁₈(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁)

发橙色荧光的亲脂性羰花青 SP-DiIC18(3) 是一种 DiI 类似物，其含有磺酸基团以提高水溶性。其在水中发弱荧光，但在掺入膜中后会发出极其明亮且光稳定的荧光。此类 DiI 类似物中掺入的磺酸盐基团可提高水溶性。在脂质环境中，其具有超高的消光系数和较短激发态寿命了解更多信息

货号	数量
D7777	5 mg

货号 D7777

价格（CNY)

5,805.00

添加至购物车

价格（CNY)

5,805.00

添加至购物车

发橙色荧光的亲脂性羰花青 SP-DiIC₁₈(3) 是一种 DiI 类似物，其含有磺酸基团以提高水溶性。其在水中发弱荧光，但在掺入膜中后会发出极其明亮且光稳定的荧光。此类 DiI 类似物中掺入的磺酸盐基团可提高水溶性。在脂质环境中，其具有超高的消光系数和较短激发态寿命（∼1 纳秒）。添加到细胞中后，这种染料就会在细胞膜内侧向扩散。

仅供科研使用。不可用于诊断程序。

检测方法荧光

适用于（设备）荧光显微镜

数量5 mg

运输条件室温

标签类型Fluorescent Dye

产品类型SP-DiIC18(3)

亚细胞定位细胞膜&脂质, Lipids

Unit SizeEach

内容与储存

室温避光储存。

常见问题解答 (FAQ)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。这是什么原因所致？

由于这些染料插入脂质膜，任何对膜的破坏都将导致染料流失。这包括用Triton X-100之类的去垢剂或是甲醇之类的有机溶剂通透。通透是胞内抗体标记所必需的，但它会导致染料流失。相反，CFDA SE之类活性染料能够共价连接到细胞组分，因此可以在固定和通透之后更好的保留。

亲脂性示踪剂跨膜转运需要多久？FAST亲脂性染料能快多少？

转运过程相当缓慢：在活体组织中大约6 mm/天，在固定组织中更慢。亲脂性羰花青示踪剂从开始给样处扩散到神经元末端，可能需要数天甚至数周的时间。FAST DIO和DiI类似物（具有不饱和烷基尾）可以提高50％左右的转运速度。

我应该使用哪种亲脂性示踪剂（DiO、DiI、DiD等）？

请选择与您现有的激发光源和发射滤光片组/通道兼容的染料。固体、糊和晶体形式的染料可直接施加到组织中的神经元。如需标记培养的细胞或进行显微注射，可采用溶液或固态形式的亲脂性染料。

我想标记两种细胞群体然后进行细胞融合实验，哪种试剂最适合来成像？

亲脂性花青染料非常适合此类测定，因为它们会掺入到细胞膜中，随着细胞融合之后共用细胞膜，染料也随之共享。例如，一个细胞群体可以用DiI（橙红色）进行标记，而另一细胞群可以用DIO（绿色）进行标记，当细胞融合时合并的颜色为黄色（使用双带通滤光片成像）。

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all SP-DiIC₁₈(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁) FAQs

引用和文献 (15)

引用和文献

Abstract

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors.

Authors:Wang TY, Sen A, Behie LA, Kallos MS,

Journal:Brain Res

PubMed ID:16859652

'Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson''s disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can ... More

Identification of a neural stem cell in the adult mammalian central nervous system.

Authors:Johansson CB, Momma S, Clarke DL, Risling M, Lendahl U, Frisén J

Journal:Cell

PubMed ID:9989494

'New neurons are continuously added in specific regions of the adult mammalian central nervous system. These neurons are derived from multipotent stem cells whose identity has been enigmatic. In this work, we present evidence that ependymal cells are neural stem cells. Ependymal cells give rise to a rapidly proliferating cell ... More

Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.

Authors:Daubeuf S, Bordier C, Hudrisier D, Joly E,

Journal:Cytometry A

PubMed ID:19051238

Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More

Evidence for neurogenesis in the adult mammalian substantia nigra.

Authors:Zhao M, Momma S, Delfani K, Carlen M, Cassidy RM, Johansson CB, Brismar H, Shupliakov O, Frisen J, Janson AM

Journal:Proc Natl Acad Sci U S A

PubMed ID:12792021

New neurons are generated from stem cells in a few regions of the adult mammalian brain. Here we provide evidence for the generation of dopaminergic projection neurons of the type that are lost in Parkinson's disease from stem cells in the adult rodent brain and show that the rate of ... More

Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors.

Authors:Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M

Journal:Cancer Res

PubMed ID:12097260

Molecules that physiologically control cell proliferation are often produced locally in tissues and are rapidly destroyed when they enter circulation. This allows local effects while avoiding interference with other systems. Unfortunately, it also limits the therapeutic use of these molecules via systemic delivery. We here demonstrate that, for the purpose ... More

View all SP-DiIC₁₈(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁) citations

SP-DiIC18(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁)

常见问题解答 (FAQ)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。 这是什么原因所致？

亲脂性示踪剂跨膜转运需要多久？FAST亲脂性染料能快多少？

我应该使用哪种亲脂性示踪剂（DiO、DiI、DiD等）？

我想标记两种细胞群体然后进行细胞融合实验，哪种试剂最适合来成像？

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

引用和文献 (15)

SP-DiIC₁₈(3) (1,1'-双十八烷基-6,6'-二(4-磺苯基)-3,3,3',3'-四甲基吲哚菁)

我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。这是什么原因所致？