EnzChek™ 弹性蛋白酶检测试剂盒
EnzChek™ 弹性蛋白酶检测试剂盒
Invitrogen™

EnzChek™ 弹性蛋白酶检测试剂盒

EnzChek 弹性蛋白酶检测试剂盒提供了一种灵敏、便利、快速的荧光测定方法,可用于在经过纯化的酶体系以及细胞/组织裂解物中测量弹性蛋白酶或其他蛋白酶活性,或用于以高通量形式筛选抑制剂。EnzChek 试剂盒中的底物是使用我们的 BODIPY-FL 标记的 DQ 弹性蛋白偶联物,其经过高度标记,以至于荧光信号被淬灭,直到酶消化后才会产生具有强烈荧光的片段了解更多信息
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货号数量
E12056600 Assays
货号 E12056
价格(CNY)
6,683.00
Each
添加至购物车
数量:
600 Assays
价格(CNY)
6,683.00
Each
添加至购物车
EnzChek 弹性蛋白酶检测试剂盒提供了一种灵敏、便利、快速的荧光测定方法,可用于在经过纯化的酶体系以及细胞/组织裂解物中测量弹性蛋白酶或其他蛋白酶活性,或用于以高通量形式筛选抑制剂。EnzChek 试剂盒中的底物是使用我们的 BODIPY-FL 标记的 DQ 弹性蛋白偶联物,其经过高度标记,以至于荧光信号被淬灭,直到酶消化后才会产生具有强烈荧光的片段。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光强度
数量600 Assays
运输条件室温
底物属性基于蛋白的底物
底物类型弹性蛋白酶底物
目标酶弹性蛋白酶
适用于(应用)弹性蛋白酶检测
产品线EnzChek
产品类型弹性蛋白酶检测
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

引用和文献 (9)

引用和文献
Abstract
Genetic and biochemical characterization of PrtA, an RTX-like metalloprotease from Photorhabdus.
Authors:Bowen DJ, Rocheleau TA, Grutzmacher CK, Meslet L, Valens M, Marble D, Dowling A, Ffrench-Constant R, Blight MA
Journal:Microbiology
PubMed ID:12777498
'Proteases play a key role in the interaction between pathogens and their hosts. The bacterial entomopathogen Photorhabdus lives in symbiosis with nematodes that invade insects. Following entry into the insect, the bacteria are released from the nematode gut into the open blood system of the insect. Here they secrete factors ... More
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Authors:Frederiks WM, Mook OR
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some ... More
Alveolar wall apoptosis causes lung destruction and emphysematous changes.
Authors:Aoshiba K, Yokohori N, Nagai A
Journal:Am J Respir Cell Mol Biol
PubMed ID:12707011
'Pulmonary emphysema is characterized by alveolar wall destruction and airspace enlargement. Recent evidence indicates that epithelial or endothelial apoptosis may be involved in the pathogenesis of emphysema. Here, we describe the induction of emphysematous changes, including airspace enlargement, alveolar wall destruction, and enhanced lung distensibility, in mice receiving a single ... More
A zinc metalloprotease inhibitor, Inh, from the insect pathogen Photorhabdus luminescens.
Authors:Valens M, Broutelle AC, Lefebvre M, Blight MA
Journal:Microbiology
PubMed ID:12177336
The entomopathogen Photorhabdus luminescens secretes many proteins during the late stages of insect larvae infection and during in vitro laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of P. luminescens. PrtA is secreted via a classical type I secretory pathway ... More
Changes in neutrophil functions in astronauts.
Authors:Kaur I, Simons ER, Castro VA, Mark Ott C, Pierson DL
Journal:Brain Behav Immun
PubMed ID:15265537
Exploration class human spaceflight missions will require astronauts with robust immune systems. Innate immunity will be an essential element for the healthcare maintenance of astronauts during these lengthy expeditions. This study investigated neutrophil phagocytosis, oxidative burst, and degranulation of 25 astronauts after four space shuttle missions and in nine healthy ... More