收到后,试剂盒应在 -20°C 条件下避光保存。20X TE 缓冲液和 lambda DNA 标准品最好在 4°C 条件下储存;但是,两者均可长期冷冻保存。如果储存得当,试剂盒组分应该能够保持稳定至少 6 个月。开瓶前,首先让试剂恢复至室温。
常见问题解答 (FAQ)
With the EnzChek Reverse Transcriptase Assay Kit, how do I expand the dynamic range?
To expand the upper end of the PicoGreen’s dynamic range, simply increase dye concentration. By doubling the concentration, the dynamic range will double, etc. Keep in mind, however that increasing the dye concentration sacrifices the lower end of the assay due to increasing background fluorescence.
Does the assay EnzChek Reverse Transcriptase Assay Kit (Cat. No. E22064) work on retroviruses other than HIV?
The assay should work with any reverse transcriptase.
Note: This is a solution reaction, so the reverse transcriptase needs to be available in solution to work, not encapsulated.