引用和文献 (4)

Invitrogen™

EnzChek™ 逆转录酶检测试剂盒

测量逆转录酶活性

货号	数量
E22064	1000 次检测

货号 E22064

价格（CNY)

8,194.00

1000 次检测

价格（CNY)

8,194.00

The EnzChek Reverse Transcriptase Assay Kit is a convenient, efficient, and inexpensive assay for measuring reverse transcriptase activity and screening reverse transcriptase inhibitors. In less than an hour, samples can be read in a fluorometer or microplate reader with filter sets appropriate for fluorescein (FITC), GFP, or Alexa Fluor 488.

EnzChek™ 逆转录酶检测试剂盒是一种便利、高效且便宜的逆转录酶活性检测方法。在 1 小时之内，研究人员即可利用配备有适合荧光素 (FITC) 的滤光片组的荧光计或微孔板读数仪对样品结果进行读取。

查看我们完整的荧光微孔板检测产品线。

•检测到低至0.02单位的 HIV-1 逆转录酶
•宽动态检测范围，可检测高达50倍的线性范围
•简单易用的测定试剂盒，适用于自动化高通量筛选应用

EnzChek™ 逆转录酶测定试剂盒使用 PicoGreen™ 试剂，可优先检测 dsDNA 或 RNA-DNA 异源双链体，而非单链核酸或游离核苷酸。在该检测中，生物样品中的逆转录酶活性利用含有长 poly(A) 模板、oligo-dT 引物和 dTTP 的混合物生成长 RNA-DNA 异源双链体。然后通过 PicoGreen™ 试剂检测形成的 RNA-DNA 异源双链体。

仅供科研使用。不可用于诊断程序。

检测方法荧光强度

发射波长523 nm

激发波长502 nm

数量1000 次检测

运输条件室温

足够用于1000 Assays

目标酶逆转录酶

适用于（应用）Enzyme and Metabolic Activity

适用于（设备）Microplate Reader

产品线EnzChek

产品类型逆转录酶检测

Unit SizeEach

内容与储存

收到后,试剂盒应在 -20°C 条件下避光保存。20X TE 缓冲液和 lambda DNA 标准品最好在 4°C 条件下储存;但是,两者均可长期冷冻保存。如果储存得当,试剂盒组分应该能够保持稳定至少 6 个月。开瓶前,首先让试剂恢复至室温。

常见问题解答 (FAQ)

With the EnzChek Reverse Transcriptase Assay Kit, how do I expand the dynamic range?

To expand the upper end of the PicoGreen’s dynamic range, simply increase dye concentration. By doubling the concentration, the dynamic range will double, etc. Keep in mind, however that increasing the dye concentration sacrifices the lower end of the assay due to increasing background fluorescence.

Does the assay EnzChek Reverse Transcriptase Assay Kit (Cat. No. E22064) work on retroviruses other than HIV?

The assay should work with any reverse transcriptase.
Note: This is a solution reaction, so the reverse transcriptase needs to be available in solution to work, not encapsulated.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4)

引用和文献

Abstract

Solution structural dynamics of HIV-1 reverse transcriptase heterodimer.

Authors:Seckler JM, Howard KJ, Barkley MD, Wintrode PL,

Journal:Biochemistry

PubMed ID:19594135

'Crystal structures and simulations suggest that conformational changes are critical for the function of HIV-1 reverse transcriptase. The enzyme is an asymmetric heterodimer of two subunits, p66 and p51. The two subunits have the same N-terminal sequence, with the p51 subunit lacking the C-terminal RNase H domain. We used hydrogen ... More

Apobec3 encodes Rfv3, a gene influencing neutralizing antibody control of retrovirus infection.

Authors:Santiago ML, Montano M, Benitez R, Messer RJ, Yonemoto W, Chesebro B, Hasenkrug KJ, Greene WC,

Journal:Science

PubMed ID:18772436

Recovery from Friend virus 3 (Rfv3) is a single autosomal gene encoding a resistance trait that influences retroviral neutralizing antibody responses and viremia. Despite extensive research for 30 years, the molecular identity of Rfv3 has remained elusive. Here, we demonstrate that Rfv3 is encoded by Apobec3. Apobec3 maps to the ... More

Importance of SARS-CoV spike protein Trp-rich region in viral infectivity.

Authors:Lu Y, Neo TL, Liu DX, Tam JP,

Journal:Biochem Biophys Res Commun

PubMed ID:18424264

SARS-CoV entry is mediated by spike glycoprotein. During the viral and host cellular membrane fusion, HR1 and HR2 form 6-helix bundle, positioning the fusion peptide closely to the C-terminal region of ectodomain to drive apposition and subsequent membrane fusion. Connecting to the HR2 region is a Trp-rich region which is ... More

Evolution of subtype C HIV-1 Env in a slowly progressing Zambian infant.

Authors:Zhang H, Hoffmann F, He J, He X, Kankasa C, Ruprecht R, West JT, Orti G, Wood C,

Journal:Retrovirology

PubMed ID:16274482

BACKGROUND: Given the high prevalence of mother to child infection, the development of a better understanding of African subtype C HIV-1 transmission and natural evolution is of significant importance. In this study, we genotypically and phenotypically characterized subtype C viruses isolated over a 67-month follow-up period from an in utero-infected ... More