表皮生长因子、四甲基罗丹明偶联物(罗丹明 EGF)
表皮生长因子、四甲基罗丹明偶联物(罗丹明 EGF)
Invitrogen™

表皮生长因子、四甲基罗丹明偶联物(罗丹明 EGF)

我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)了解更多信息
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货号数量
E348120 μg
货号 E3481
价格(CNY)
7,400.00
Each
添加至购物车
数量:
20 μg
价格(CNY)
7,400.00
Each
添加至购物车
我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)、Oregon Green™ 514 和四甲基罗丹明,使用与 EGF 直接连接的染料构建。EGF 与 Alexa Fluor™ 染料和 Texas Red™ 染料的偶联物是染料–链霉亲和素分子和生物素化 EGF 的复合物。生物素化偶联物利用生物素-XX,它含有一个长间隔臂,旨在增强探针对 EGF 受体的亲和力。

表皮生长因子偶联物规格:
• EGF 分子:53 个氨基酸,MW=6,045 Da
• 标记(激发/发射波长):四甲基罗丹明 (∼555/580 nm)
• 每个 EGF 分子上的荧光基团分子数量:1 个
•通常使用流式细胞仪、荧光显微镜或荧光计监测荧光信号


查找更多的受体结合和吞噬作用探针
我们提供多种用于研究受体介导的内吞作用的荧光标记探针,用于内吞作用和胞吐作用的膜标记物,以及检测内吞的荧光配体的方法。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节,了解有关这些产品的更多信息。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
细胞类型哺乳动物细胞
检测方法荧光
标签或染料经典染料
数量20 μg
运输条件湿冰
形式固体
产品类型表皮生长因子
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (31)

引用和文献
Abstract
Ubiquitination and proteasomal activity is required for transport of the EGF receptor to inner membranes of multivesicular bodies.
Authors:Longva KE, Blystad FD, Stang E, Larsen AM, Johannessen LE, Madshus IH
Journal:J Cell Biol
PubMed ID:11864992
'EGF, but not TGF alpha, efficiently induces degradation of the EGF receptor (EGFR). We show that EGFR was initially polyubiquitinated to the same extent upon incubation with EGF and TGF alpha, whereas the ubiquitination was more sustained by incubation with EGF than with TGF alpha. Consistently, the ubiquitin ligase c-Cbl ... More
Direct pathway from early/recycling endosomes to the Golgi apparatus revealed through the study of shiga toxin B-fragment transport.
Authors:Mallard F, Antony C, Tenza D, Salamero J, Goud B, Johannes L
Journal:J Cell Biol
PubMed ID:9817755
'Shiga toxin and other toxins of this family can escape the endocytic pathway and reach the Golgi apparatus. To synchronize endosome to Golgi transport, Shiga toxin B-fragment was internalized into HeLa cells at low temperatures. Under these conditions, the protein partitioned away from markers destined for the late endocytic pathway ... More
Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery.
Authors:Martina JA, Bonangelino CJ, Aguilar RC, Bonifacino JS
Journal:J Cell Biol
PubMed ID:11381094
'Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. Here, we report the identification of two ubiquitously expressed human proteins, stonin 1 and stonin 2, related to components of the endocytic machinery. The human stonins are homologous ... More
A novel 14-kilodalton protein interacts with the mitogen-activated protein kinase scaffold mp1 on a late endosomal/lysosomal compartment.
Authors:Wunderlich W, Fialka I, Teis D, Alpi A, Pfeifer A, Parton RG, Lottspeich F, Huber LA
Journal:J Cell Biol
PubMed ID:11266467
'We have identified a novel, highly conserved protein of 14 kD copurifying with late endosomes/lysosomes on density gradients. The protein, now termed p14, is peripherally associated with the cytoplasmic face of late endosomes/lysosomes in a variety of different cell types.In a two-hybrid screen with p14 as a bait, we identified ... More
Cytochalasins and colchicine increase the lateral mobility of human chorionic gonadotropin-occupied luteinizing hormone receptors on ovine luteal cells.
Authors:Roess DA, Niswender GD, Barisas BG
Journal:Endocrinology
PubMed ID:3257187
'To determine whether cytoskeletal proteins restrict the mobility of LH-receptor complexes occupied by hCG, we measured the rate of lateral diffusion and the fraction of mobile receptor-hormone complexes by fluorescence photobleaching recovery on luteal cells treated with microtubule and microfilament disruptors and on portions of the plasma membrane separated from ... More