Cfr10I (BsrFI) (10 U/μL)
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Thermo Scientific™

Cfr10I (BsrFI) (10 U/μL)

5' R ↓C C G G Y 3' 3' Y G G C C ↑R 5' Thermo Scientific Cfr10I (BsrFI)了解更多信息
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货号 ER0181
价格(CNY)/ 200 units
价格424.00
飞享价297.00仅限网单直购,无需促销代码
(ends 31-Dec-2024)
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价格(CNY)/ 200 units
飞享价297.00仅限网单直购,无需促销代码
(ends 31-Dec-2024)
添加至购物车
Cfr10I (BsrFI) (10 U/μL)
货号ER0181
价格(CNY)/ 200 units
飞享价297.00仅限网单直购,无需促销代码
(ends 31-Dec-2024)
-
添加至购物车
5'  R ↓C  C  G  G  Y   3' 
3'  Y  G  G  C  C ↑R   5' 

Thermo Scientific Cfr10I (BsrFI) restriction enzyme recognizes R^CCGGY sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Bse118I, BsrFI, BssAIm). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
规格
浓度10 U/μL
甲基化敏感性对 CpG 甲基化敏感, 对 Dam 甲基化不敏感, 对 Dcm 甲基化不敏感
Cfr10I (BsrFI)
兼容缓冲液独特缓冲液(10X 缓冲液 Cfr10I (BsrFI))
对热失活敏感
最佳反应温度37°C
类型 IIS RE
数量200 units
产品类型限制性内切酶
研究类别传统方法克隆
Unit Size200 units