Combined confocal and wide-field high-resolution cytometry of fluorescent in situ hybridization-stained cells.
Authors:Kozubek M, Kozubek S, Lukásová E, Bártová E, Skalníková M, Matula P, Matula P, Jirsová P, Cafourková A, Koutná I
Journal:Cytometry
PubMed ID:11598941
BACKGROUND: The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of fluorescent in situ hybridization (FISH)-stained cell nuclei using conventional wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. METHODS: ... More