FluoReporter™ 蓝色荧光测定 dsDNA 定量试剂盒，200-2,000 次测定

引用和文献 (14)

Invitrogen™

FluoReporter™ 蓝色荧光测定 dsDNA 定量试剂盒，200-2,000 次测定

FluoReporter™ 蓝色荧光 dsDNA 定量试剂盒提供了使用蓝色荧光 Hoechst 33258 核酸染色剂分析细胞 NA 的方案和试剂。使用该试剂盒可以快速对细胞DNA定量，并且所有操作均可在微孔板内完成了解更多信息

货号	数量
F2962	200 至 2000 次测定

货号 F2962

价格（CNY)

4,106.00

添加至购物车

200 至 2000 次测定

价格（CNY)

4,106.00

添加至购物车

FluoReporter™ 蓝色荧光 dsDNA 定量试剂盒提供了使用蓝色荧光 Hoechst 33258 核酸染色剂分析细胞 NA 的方案和试剂。使用该试剂盒可以快速对细胞DNA定量，并且所有操作均可在微孔板内完成。将细胞在蒸馏水中冷冻裂解，然后向裂解的细胞中加入稀释的染料溶液并测量荧光。

仅供科研使用。不可用于诊断程序。

适用于（设备）微孔板读数仪

产品线FluoReporter

产品类型dsDNA 定量测定试剂盒

数量200 至 2000 次测定

运输条件室温

检测方法荧光

产品规格96 孔板

标签或染料其他标记物或染料

Unit SizeEach

内容与储存

请避光冷藏 (2–8°C) 储存。

常见问题解答 (FAQ)

Can I use Hoechst dyes to do both DNA quantitation and cell number counting in plates?

Yes, this can be done in a 96-well plate-based format. Hoechst dyes will label all cells, live or dead (or even fixed). This requires the use of a standard curve of known cell numbers (up to 100,000 cells per well) and a standard curve using purified DNA, , up to 10 µg/mL. We offer a kit for this: FluoReporter Blue Fluorometric dsDNA Quantitation Kit, Cat. No. F2962.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (14)

引用和文献

Abstract

Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes.

Authors:Zhang JW, Tang QQ, Vinson C, Lane MD

Journal:Proc Natl Acad Sci U S A

PubMed ID:14688407

'Hormonal induction of growth-arrested 3T3-L1 preadipocytes rapidly activates expression of CCAAT/enhancer-binding protein (C/EBP) beta. Acquisition of DNA-binding activity by C/EBPbeta, however, is delayed until the cells synchronously enter the S phase of mitotic clonal expansion (MCE). After MCE, C/EBPbeta activates expression of C/EBPalpha and peroxisome proliferator-activated receptor gamma, which then ... More

In vivo imaging platform for tracking immunotherapeutic cells.

Authors:Ahrens ET, Flores R, Xu H, Morel PA

Journal:Nat Biotechnol

PubMed ID:16041364

Cellular therapeutics show great promise for the treatment of disease, but few noninvasive techniques exist for monitoring the cells after administration. Here we present a magnetic resonance imaging (MRI) technology that uses perfluoropolyether (PFPE) agents to track cells in vivo. Fluorine MRI selectively images only the labeled cells, and a ... More

ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells.

Authors:Ryten M, Dunn PM, Neary JT, Burnstock G

Journal:J Cell Biol

PubMed ID:12135987

ATP is well known for its role as an intracellular energy source. However, there is increasing awareness of its role as an extracellular messenger molecule (Burnstock, 1997). Although evidence for the presence of receptors for extracellular ATP on skeletal myoblasts was first published in 1983 (Kolb and Wakelam), their physiological ... More

5-HT2A receptor induces ERK phosphorylation and proliferation through ADAM-17 tumor necrosis factor-alpha-converting enzyme (TACE) activation and heparin-bound epidermal growth factor-like growth factor (HB-EGF) shedding in mesangial cells.

Authors:Göoz M, Göoz P, Luttrell LM, Raymond JR

Journal:J Biol Chem

PubMed ID:16737974

In this study, we present multiple lines of evidence to support a critical role for heparin-bound EGF (epidermal growth factor)-like growth factor (HB-EGF) and tumor necrosis factor-alpha-converting enzyme (TACE) (ADAM17) in the transactivation of EGF receptor (EGFR), ERK phosphorylation, and cellular proliferation induced by the 5-HT(2A) receptor in renal mesangial ... More

Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.

Authors:

Journal:FASEB J

PubMed ID:32297675

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