Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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货号化学名称或材料颜色
F519LGC Buffer PackColorless
F518LHF Buffer PackColorless
F539LGC Buffer PackGreen
货号 F519L
价格（CNY)
300.00
Each
添加至购物车
化学名称或材料:
GC Buffer Pack
颜色:
Colorless
请求批量或定制报价
价格（CNY)
300.00
Each
添加至购物车
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
仅供科研使用。不可用于诊断程序。
规格
化学名称或材料GC Buffer Pack
颜色Colorless
描述Phusion™ GC 缓冲液套装
包括Phusion GC 缓冲液包包括：
• 5X Phusion GC 缓冲液（1X 缓冲液含 1.5 mM MgCl2）
• 50 mM MgCl2
• DMSO

在 -20°C 下储存。
适用于（应用）常规 PCR
数量6 mL
Unit SizeEach