Phusion 血液直接 PCR 试剂盒
Phusion 血液直接 PCR 试剂盒
Thermo Scientific™

Phusion 血液直接 PCR 试剂盒

Thermo Scientific Phusion 血液直接 PCR 试剂盒开发用于从全血中扩增 DNA。无需在 PCR 前进行单独的了解更多信息
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货号反应次数
F547S100 次反应
F547L500 次反应
货号 F547S
价格(CNY)
946.00
飞享价
Ends: 31-Dec-2025
1,350.00
共减 404.00 (30%)
Each
添加至购物车
反应次数:
100 次反应
请求批量或定制报价
价格(CNY)
946.00
飞享价
Ends: 31-Dec-2025
1,350.00
共减 404.00 (30%)
Each
添加至购物车
Thermo Scientific Phusion 血液直接 PCR 试剂盒开发用于从全血中扩增 DNA。无需在 PCR 前进行单独的 DNA 纯化步骤。改良型 Phusion 热启动 II 高保真 DNA 聚合酶对血液中发现的 PCR 抑制剂表现出高度耐受性,即使在含 40% 全血的样品中也能保留聚合酶活性。

这种 DNA 聚合酶在校正性方面表现出非凡的准确度,并使 Phusion 血液直接 PCR 试剂盒成为要求苛刻的应用(如克隆)的较佳选择之一。Phusion 血液直接 PCR 试剂盒具有多功能性,还允许分析不同物种的血样,以及使用不同的抗凝剂或储存条件分析血样。
产品优势:

•无需耗时且昂贵的 DNA 纯化步骤
•简单的方案需要极短的手动操作时间
• 极短的 PCR 方案时间
• 改良型 Phusion 热启动 II 高保真 DNA 聚合酶能够获得极高的特异性产物得率

Phusion DNA 聚合酶的较佳退火温度可能与基于 Taq 的聚合酶显著不同。

为了获得较佳结果,首先使用我们的 Tm 计算器准确计算您的 Tm。
仅供科研使用。不可用于诊断程序。
规格
热启动内置热启动
反应次数100 次反应
突出端平末端
聚合酶Phusion 血液 II DNA 聚合酶
产品类型Direct PCR 试剂盒
数量100 × 20 μL 反应
反应形式分离组分
样品类型血液
运输条件湿冰或干冰
原始材料DNA
最大浓度2 U/μL
高 GC PCR 扩增效果
PCR 方法直接 PCR
反应速度快速
Unit SizeEach
内容与储存
包含:
• 40 µL Phusion 血液 II DNA 聚合酶
• 1.25 mL 2X Phusion Blood PCR 缓冲液(包括 dNTP 和 MgCl2
• 40 µL 通用型对照引物混合物
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO

在 -20°C 下储存。

常见问题解答 (FAQ)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

What is the highest blood concentration possible in the reaction when using Phusion Blood Direct PCR Master Mix and Kit?

We recommend using blood concentration up to 20%; however, we had successful amplifications with up to 40% of blood in the reaction mix. In some cases optimization may be required to achieve this.