该预混液采用 Phusion U 热启动 DNA 聚合酶,这是一种使用融合技术开发的高性能酶。与 Phusion 系列的其他酶类似,Phusion U DNA 聚合酶在每次结合中引入了更多的核苷酸。这样可以大大缩短延伸步骤的时间,但 PCR 产物的产量更高,从而减少了整个实验流程的时间。显著提高的持续合成能力还可实现对困难靶标的成功多重 PCR,例如高 GC 含量模板或纯度不佳的模板。Phusion U 多通路 PCR 预混液高度耐受多种 PCR 抑制剂,甚至适用于未纯化样品(如血液或血清)。
使用 Phusion DNA 聚合酶 Phusion DNA 聚合酶的退火温度设计规则和许多普通 DNA 聚合酶(如 Taq DNA 聚合酶)不一样。为了获得最佳结果,请使用 www.thermofisher.cn/tmcalculator 上的 Tm 计算器。
仅供科研使用。不可用于诊断程序。
规格
颜色Colorless
保真度(相对于 Taq)25X
热启动内置热启动
反应次数100 次反应
突出端平末端
聚合酶Phusion U 热启动 DNA 聚合酶
产品类型多重 PCR 预混液
数量100 × 50 μL 反应
反应形式SuperMix 或预混液
运输条件干冰
尺寸(最终产品)2.5 kb 或更小
最大浓度2X
适用于(应用)Hot-start PCR, Genotyping
高 GC PCR 扩增效果中
反应速度快速
Unit SizeEach
内容与储存
Phusion U 多重 PCR 预混液含有: • Phusion U 热启动 DNA 聚合酶 • 2X 反应缓冲液 • 3 mM MgCl2 • 400 μ M 的 dATP、dTTP、dCTP 和 dGTP 该预混液随附无核酸酶水。 -20° C 储存。
常见问题解答 (FAQ)
How much blood can be added directly to multiplex PCR reaction using Phusion U Multiplex PCR Master Mix?
For direct multiplex PCR without prior DNA purification, add up to 4 µL of whole blood to 50 µL final reaction volume.
Can Phusion U Multiplex PCR Master Mix be stored at +4 degrees C?
Yes, Phusion U Multiplex PCR Master Mix can be stored at +4 degrees C for up to 3 months with no effect on performance.
What is the limit of amplicon size using Phusion U Multiplex PCR Master Mix?
Phusion U Multiplex PCR Master Mix is designed for simultaneous amplification of targets up to 2.5 kb.
How many fragments can be amplified in a single reaction using Phusion U Multiplex PCR Master Mix?
We have demonstrated successful simultaneous amplification of 24 DNA fragments.
What are the Phusion U Multiplex PCR Master Mixes and what is the purpose of the Green format?
Thermo Scientific Phusion U Multiplex PCR Master Mixes (Cat. No. F562S, F564S, F562L, F564L) are ready-to-use, 2X end-point PCR master mixes designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations. Phusion U Multiplex PCR Master Mixes contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization.
The Green format of the Phusion U Multiplex PCR Master Mixes (Cat. No. F564S, F564L) further simplifies the workflow: it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.