FIX & PERM™ 细胞透化试剂盒

您应该加入约100 µL的细胞。为此，方法之一是分装正确数量的细胞（10E6），然后离心去上清。残留体积大约为100 µL。

可以。通常情况下，您需要延长在通透缓冲液（试剂B）中的孵育时间，以下为两份相关的文献：

•Millard I, Degrave E, Philippe M et al. (1998) Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating. Clin Chem 44:2320–2330.
•Parker JE, Mufti GJ, Rasool F et al. (2000) The role of apoptosis, proliferation, and the Bcl-2–related proteins in the myelodysplastic syndromes and acute myeloid leukemia secondary to MDS. Blood 96:3932–3938.

可以，以下为两份相关的文献：

Haranaga S, Yamaguchi H, Friedman H et al.(2001) Chlamydia pneumoniae infects and multiplies in lymphocytes In vitro. Infect Immun 69:7753–7759.
Al-Mufti R, Hambley H, Albaiges G et al. (2000) Increased fetal erythroblasts in women who subsequently develop pre-eclampsia. Hum Reprod 15:1624–1628.

会。在流式细胞术应用中，FIX & PERM细胞通透试剂盒可用于固定和通透悬浮细胞，从而使抗体可以接近到胞内靶标且不降低其散射特性。此试剂盒经验证可用于细胞系、全血和骨髓。除了固定和通透细胞外，该试剂盒还可裂解红细胞。其可作为通用标记试剂（GPR），可用于临床应用，同时也可广泛用于实验室研究。

Yes, this product is compatible with other conjugated antibodies, outside of FITC and PE.