Image-iT™ Green 缺氧监测试剂
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Image-iT™ Green 缺氧监测试剂
Invitrogen™

Image-iT™ Green 缺氧监测试剂

Image-iT Green缺氧监测试剂是一种新型、可固定的荧光化合物,用于测定活细胞中的缺氧情况。活细胞在具有正常氧气浓度的环境中不发荧光,而当氧水平降低时会发出荧光。当细胞/组织恢复至正常氧水平时,Image-iT Green 缺氧监测试剂可维持其荧光,从而使细胞/组织的荧光信号损失极小。Image-iT Green 缺氧试剂的特点包括:•在低氧环境中发出荧光可以指示活细胞缺氧•固定—细胞/组织恢复正常氧水平时,维持荧光信号•易于使用—仅添加到细胞培养基中并成像了解更多信息
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货号数量
I148341 小瓶
I148335 小瓶
货号 I14834
价格(CNY)
1,259.00
Online Exclusive
Ends: 31-Dec-2025
1,695.00
共减 436.00 (26%)
Each
添加至购物车
数量:
1 小瓶
价格(CNY)
1,259.00
Online Exclusive
Ends: 31-Dec-2025
1,695.00
共减 436.00 (26%)
Each
添加至购物车
Image-iT Green缺氧监测试剂是一种新型、可固定的荧光化合物,用于测定活细胞中的缺氧情况。活细胞在具有正常氧气浓度的环境中不发荧光,而当氧水平降低时会发出荧光。当细胞/组织恢复至正常氧水平时,Image-iT Green 缺氧监测试剂可维持其荧光,从而使细胞/组织的荧光信号损失极小。

Image-iT Green 缺氧试剂的特点包括:
•在低氧环境中发出荧光可以指示活细胞缺氧
•固定—细胞/组织恢复正常氧水平时,维持荧光信号
•易于使用—仅添加到细胞培养基中并成像

Image-iT Green 缺氧监测试剂是一种可渗透活细胞的荧光化合物,在低氧浓度环境中发出荧光。这些特性使其成为在缺氧条件中检测细胞和组织的非常有用的工具。Image-iT Green 缺氧监测试剂是一种非常灵敏的氧检测器。与仅对极低氧水平产生反应的哌莫硝唑加合物不同,当大气氧水平低于 5% 时,Image-iT Green 缺氧监测试剂开始发出荧光。它可快速响应这种环境,并且在氧水平恢复正常后仍会持续荧光。这些特性使 Image-iT Green 缺氧监测试剂成为检测肿瘤、3D 培养物、球状体、神经元等周围缺氧条件的理想工具。

仅供科研使用。不可用于诊断程序。
规格
数量1 小瓶
试剂类型缺氧试剂
运输条件经批准可置于湿冰或干冰上运输
产品线Image-iT™
产品类型试剂名称
Unit SizeEach
内容与储存
在 -5 至 -30°C 下储存。避光。
  • 接收后至少可稳定保存 6 个月。
  • 常见问题解答 (FAQ)

    Can Image-it Green Hypoxia Reagent be used in cells expressing fluorescent proteins?

    Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Our Image-iT Green Hypoxia Reagent is already highly fluorescent before using the product. What could have caused this?

    This could be due to storage of the product under anaerobic or low oxygen conditions. The Image-iT Green Hypoxia Reagent increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Image-iT Green Hypoxia Reagent.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    May I include a total cell stain (to stain all cells) along with Image-iT Hypoxia Reagent?

    You may use cell-permeable nuclear counterstains or surface labels, but avoid any general cytoplasmic stains that may interfere with the emission of the hypoxia reagents.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.