Image-iT™ TMRM 试剂(线粒体膜电位指示剂)
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Image-iT™ TMRM 试剂(线粒体膜电位指示剂)
Invitrogen™

Image-iT™ TMRM 试剂(线粒体膜电位指示剂)

正常线粒体膜维持着细胞器内外之间的电势差异,称为膜电位。四甲基罗丹明甲基酯(Tetramethylrhodamine, methyl ester,以下简称 TMRM)是一种能透过细胞的染料,可在具有完整膜电位的活性线粒体中累积。如果细胞线粒体正常且发挥功能,会发出荧光信号。一旦线粒体膜电位丢失,TMRM 不再累积,荧光信号变弱或消失了解更多信息
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货号数量
I343615 x 100 μL
货号 I34361
价格(CNY)
2,010.00
飞享价
Ends: 31-Dec-2025
2,723.00
共减 713.00 (26%)
Each
添加至购物车
数量:
5 x 100 μL
价格(CNY)
2,010.00
飞享价
Ends: 31-Dec-2025
2,723.00
共减 713.00 (26%)
Each
添加至购物车
正常线粒体膜维持着细胞器内外之间的电势差异,称为膜电位。四甲基罗丹明甲基酯(Tetramethylrhodamine, methyl ester,以下简称 TMRM)是一种能透过细胞的染料,可在具有完整膜电位的活性线粒体中累积。如果细胞线粒体正常且发挥功能,会发出荧光信号。一旦线粒体膜电位丢失,TMRM 不再累积,荧光信号变弱或消失。TMRM 信号可通过荧光显微镜、流式细胞分析、细胞分选、高通量筛选和高内涵分析进行检测。

•对于线粒体膜电位(不固定)的活细胞动态研究
• 可使用 RFP/TRITC 滤光片(吸收峰:548 nm,发射峰:574 nm)
• 可用绿色、深红色、紫色和蓝色发射染料(如 GFP、DAPI、Hoechst、HCS LipidTOX 和 LysoTracker Deep Red)进行多色检测

仅供科研使用。不可用于诊断程序。
规格
数量5 x 100 μL
运输条件湿冰
颜色橙色
产品线Molecular Probes™
产品类型试剂名称
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

For a final staining solution, we can add TMRM directly into cell growth medium. Can this media contain serum?

Yes, you may add TMRM to media with serum. Serum components may bind dye non-specifically so you may need a higher final working concentration for staining samples in media containing serum relative to staining the same cells in a non-serum media or buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is included in the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is a ready-to-use 1,000X concentrated stock solution of the TMRM dye. It contains 5 vials of 100 µM of TMRM in neat DMSO, with 100 µL volume in each vial. TMRM powder (Cat No. T668) is not included in this kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.