Image-iT™ 细胞涂覆试剂盒
Image-iT™ 细胞涂覆试剂盒
Invitrogen™

Image-iT™ 细胞涂覆试剂盒

Image-iT 细胞全景绘制试剂盒是一组试剂,可用于通过“涂覆”不同的细胞成分来检查细胞健康/细胞毒性。与高内涵成像仪(如 CellInsight 平台)结合使用时,这些试剂可以提供细胞状态的表型读数。本试剂盒在 Bray 等人的发表成果的基础上制作了解更多信息
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货号数量
I65000足够用于两块384或96孔板
I65500足够用于十块384或96孔板
货号 I65000
价格(CNY)
3,746.00
Each
添加至购物车
数量:
足够用于两块384或96孔板
价格(CNY)
3,746.00
Each
添加至购物车
Image-iT 细胞全景绘制试剂盒是一组试剂,可用于通过“涂覆”不同的细胞成分来检查细胞健康/细胞毒性。与高内涵成像仪(如 CellInsight 平台)结合使用时,这些试剂可以提供细胞状态的表型读数。本试剂盒在 Bray 等人的发表成果的基础上制作。(1) 使用多通路荧光染料描述细胞的形态特征。

本试剂盒包含以下试剂:
•Hoechst 34580、三盐酸盐、三水合物
• 伴刀豆球蛋白 A、Alexa Fluor 488 偶联物
• 麦胚凝集素、Alexa Fluor 555 偶联物
• Alexa Fluor 568 鬼笔环肽
• SYTO 14 绿色荧光核酸染色剂
• MitoTracker Deep Red FM

参考文献
1.Bray MA, Singh S, Han H, Davis CT, Borgeson B, Hartland C, Kost-Alimova M, Gustafsdottir SM, Gibson CC, Carpenter AE.Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes.Nat Protoc.2016 Sep;11(9):1757-74.

规格
产品规格试剂盒
标签类型Fluorescent Dye
数量足够用于两块384或96孔板
子细胞定位Cytoskeleton , Plasma Membrane, Golgi, Mitochondria, Cytoplasm, Nucleus, Endoplasmic Reticulum, Nucleolus
颜色Multicolor
适用于(应用)High-content Screening (HCS), Morphological Cell Profiling, Multiparameter Screening, Fluorescent Labeling, 3D Labeling
适用于(设备)Fluorescence Microscope , High Content Analysis Instrument, CellInsight CX7 Pro, CellInsight CX7 LZR Pro
产品线Image-iT
产品类型细胞全景绘制试剂盒
Unit SizeEach
内容与储存
足够用于两块384或96孔板

常见问题解答 (FAQ)

How many plates can I stain using the Image-iT Cell Painting Kit (Cat. No. I65000)?

The Image-iT Cell Painting Kit (Cat. No. I65000) contains sufficient reagents to stain 2 x 96‑well plates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (2)

引用和文献
Abstract
Cell Painting: a decade of discovery and innovation in cellular imaging.
Authors:Seal S,Trapotsi MA,Spjuth O,Singh S,Carreras-Puigvert J,Greene N,Bender A,Carpenter AE
Journal:Nature methods
PubMed ID:39639168
Modern quantitative image analysis techniques have enabled high-throughput, high-content imaging experiments. Image-based profiling leverages the rich information in images to identify similarities or differences among biological samples, rather than measuring a few features, as in high-content screening. Here, we review a decade of advancements and applications of Cell Painting, a ... More
Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes.
Authors:Bray MA,Singh S,Han H,Davis CT,Borgeson B,Hartland C,Kost-Alimova M,Gustafsdottir SM,Gibson CC,Carpenter AE
Journal:Nature protocols
PubMed ID:27560178
In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, a morphological profiling assay multiplexing six fluorescent dyes imaged in five channels, to ... More