iBlot™ 2 Transfer Stacks, PVDF, mini

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This mini-size stack (8 x 8 cm) can be used with one mini-size gel.

PVDF membrane
The PVDF membrane (0.2-μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 μg/cm².

每个转印膜组在凝胶基质中均包含一个铜涂层电极以及适当的阴极和阳极缓冲液，以快速、可靠转印蛋白质。

• 保质期更久
• 精简型紧凑包装
• 完全兼容 iBlot 2 凝胶转印装置

PVDF 膜特性

• 0.2μm 孔径，低荧光
• 比硝酸纤维素结合能力更高
• 已预先活化，无需进行甲醇预处理即可使用
• 与常用的检测方法兼容，例如染色，免疫检测，荧光或放射性标记
• 通过疏水相互作用而结合蛋白
• 蛋白结合容量：240μ/cm²

• 常规尺寸 (13 x 8.3 cm) 可用于所有 Novex 中型凝胶（或其他中型凝胶类型）或两块 Novex 小型凝胶（或其他小型凝胶类型）以及 E-PAGE™ 48 或 96 凝胶
• 该小型尺寸 (8 x 8 cm) 可用于所有 Novex 小型凝胶（或其他小型凝胶类型）

蛋白、表达、分离和分析、Western 印迹