**********************备选产品:试用 TaqMan Fast Advanced 预混液,这是我们较高性能的探针预混液。有了 TaqMan Fast Advanced 预混液,我们可以充分发挥了解更多信息
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货号
数量
K0331
250 × 20 μL 反应
K0334
5000 × 20 μL 反应
2 选项
货号 K0331
价格(CNY)
2,340.00
Each
添加至购物车
数量:
250 × 20 μL 反应
价格(CNY)
2,340.00
Each
添加至购物车
********************** 备选产品:试用 TaqMan Fast Advanced 预混液,这是我们较高性能的探针预混液。有了 TaqMan Fast Advanced 预混液,我们可以充分发挥 Luminaris Color Probe qPCR 预混液的作用,并为您的基因表达分析带来更多的实用功能。
最大浓度2X(Luminaris Color Probe 高浓度 ROX qPCR 预混液)、40X(黄色样品缓冲液)
检测方法引物-探针
适用于(应用)基因表达, 基因分型, siRNA 验证
高 GC PCR 扩增效果高
PCR 方法qPCR
反应速度标准
Unit SizeEach
内容与储存
包含: •2 x 1.25 mL Luminaris 彩色探针高浓度 ROX qPCR 预混液 (2X) 包含热启动 Taq DNA 聚合酶、UDG、dNTP(以及 dUTP),溶于含蓝色染料的优化 PCR 缓冲液,足以进行 250 x 20 µL 反应。该预混液包含高浓度 ROX 惰性参比染料,适用于特定实时平台。 •1 x 1.25 mL 40X 黄色样品缓冲液 •2 x 1.25 mL 无核酸酶水
储存于 -20°C 条件下。
常见问题解答 (FAQ)
Does dUTP in Luminaris qPCR master mix influence qPCR efficiency?
Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.
What enzyme is used in Luminaris qPCR Master Mixes?
Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.
Can I prepare a qPCR plate in advance and store it for later use when using Luminaris qPCR Master Mixes?
When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.