引用和文献 (4)

Thermo Scientific™

GeneJET 质粒大提试剂盒

Name: GeneJET 质粒大提试剂盒
SKU: K0491

Thermo Scientific GeneJET 质粒大提试剂盒足够用于 25 次制备，采用便捷离心柱形式的硅胶膜技术。每次制备可回收高达 750 μg 的高拷贝质粒了解更多信息

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货号	反应次数
K0491	10 次制备
K0492	25 次制备

2 选项

货号 K0491

价格（CNY)

反应次数:

10 次制备

Thermo Scientific GeneJET 质粒大提试剂盒足够用于 25 次制备，采用便捷离心柱形式的硅胶膜技术。每次制备可回收高达 750 μg 的高拷贝质粒 DNA，可用于各种分子生物学程序，例如限制性核酸内切酶酶切、PCR、克隆、转化、自动测序、体外转录和稳健细胞系转染。

产品优势

•高效—在 250 mL 细菌培养物中获得高达 750 μg 的高质量质粒 DNA
•高浓度的纯化 DNA—> 400 ng/μL
• 便捷—离心柱形式、离心或真空方案
•快速—程序需 60 分钟，比使用重力流柱快得多

应用

•分离的质粒 DNA 适用于所有常规分子生物学程序，包括：
•稳健细胞系转染
• FastDigest 限制性内切酶酶切
•自动荧光测序
•PCR
•克隆
•转化
•体外转录

仅供科研使用。不可用于诊断程序。

规格

色谱柱类型离心柱

最大浓度400 ng/µL

适用于（应用）实时荧光定量 PCR (qPCR)、转染、克隆、测序、体外转录、转化、核酸标记、PCR

高通量能力不兼容高通量应用（手动）

反应次数10 次制备

制备规模> 200 μg（大规模）质粒 DNA

产品线GeneJET

产品类型质粒 MaxiPrep 试剂盒

数量10 次制备

样品类型细菌

规格Maxi

靶标质粒 DNA

测试时间60 min。

产品规格离心柱

分离技术硅胶柱

Unit SizeEach

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

View all GeneJET 质粒大提试剂盒 FAQs

引用和文献 (4)

引用和文献

Abstract

Biochemical characterization of the human ubiquitous glucose-6-phosphatase in neutrophil granulocytes.

Authors:Lédeczi Z,Németh K,Kardon T

Journal:FEBS open bio

PubMed ID:39548727

Glucose‐6‐phosphatase‐β (G6PC3) is a ubiquitous phosphatase present in the endoplasmic reticulum, which, unlike G6PC1, is not responsible for maintaining blood glucose level under starvation. Recently, G6PC3 has been shown to play an important role in neutrophil granulocytes, eliminating the toxic metabolite 1,5‐anhydroglucitol‐6‐phosphate. The present study aimed to look for alternative ... More

Universal Glycosyltransferase Continuous Assay for Uniform Kinetics and Inhibition Database Development and Mechanistic Studies Illustrated on ST3GAL1, C1GALT1, and FUT1.

Authors:Nashed A,Naidoo KJ

Journal:ACS omega

PubMed ID:38645360

[Image: see text] Chemical systems glycobiology requires experimental and computational tools to make possible big data analytics benefiting genomics and proteomics. The impediment to tool development is that the nature of glycan construction and mutation is not template driven but rests on cooperative glycosyltransferase (GT) catalytic synthesis. What is needed ... More

Inhibition of a lower potency target drives the anticancer activity of a clinical p38 inhibitor.

Authors:Bhattacharjee D,Bakar J,Chitnis SP,Sausville EL,Ashtekar KD,Mendelson BE,Long K,Smith JC,Heppner DE,Sheltzer JM

Journal:Cell chemical biology

PubMed ID:37827156

The small-molecule drug ralimetinib was developed as an inhibitor of the p38α mitogen-activated protein kinase, and it has advanced to phase 2 clinical trials in oncology. Here, we demonstrate that ralimetinib resembles EGFR-targeting drugs in pharmacogenomic profiling experiments and that ralimetinib inhibits EGFR kinase activity in vitro and in cellulo. ... More

Low dietary fiber intake impairs small intestinal Th17 and intraepithelial T cell development over generations.

Authors:Royer CJ,Rodriguez-Marino N,Yaceczko MD,Rivera-Rodriguez DE,Ziegler TR,Cervantes-Barragan L

Journal:Cell reports

PubMed ID:37768824

Dietary fiber strongly impacts the microbiota. Here, we show that a low-fiber diet changes the small intestinal (SI) microbiota and impairs SI Th17, TCRαβ( )CD8αβ( ) and TCRαβ( )CD8αα( ) intraepithelial T cell development. We restore T cell development with dietary fiber supplementation, but this defect becomes persistent over generations ... More