GeneJET 植物 RNA 纯化试剂盒
We have updated the storage temperature of the purification columns in this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
GeneJET 植物 RNA 纯化试剂盒
Thermo Scientific™

GeneJET 植物 RNA 纯化试剂盒

Thermo Scientific GeneJET 植物 RNA 纯化小提试剂盒专为从各种植物物种和组织类型中快速高效地纯化高质量的总 RNA 而设计。试剂盒采用便捷离心柱形式的硅胶膜技术,无需使用昂贵的树脂、有毒的苯酚-氯仿提取或耗时的酒精沉淀了解更多信息
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货号数量
K0802250 次制备
K080150 次制备
货号 K0802
价格(CNY)
-
数量:
250 次制备
Thermo Scientific GeneJET 植物 RNA 纯化小提试剂盒专为从各种植物物种和组织类型中快速高效地纯化高质量的总 RNA 而设计。

试剂盒采用便捷离心柱形式的硅胶膜技术,无需使用昂贵的树脂、有毒的苯酚-氯仿提取或耗时的酒精沉淀。细胞裂解后,标准程序所需时间小于 20 分钟。纯化的高质量 RNA 可用于多种下游应用,例如 RT-PCR、 RT-qPCR、Northern 印迹和其他 RNA 分析。不同物种、组织和组织样品年龄之间的 RNA 产量各不相同。有关不同来源的典型总 RNA 产量,请参见表 1。

产品优势

快速—细胞裂解后在 20 分钟内完成程序
便捷—方案不包括易堵塞的裂解物过滤步骤
高得率—从 50 mg 植物样品中可获得高达 65 μg 的高纯度和高完整性 RNA
兼容可用于多种样品(各种植物的叶片、根部、芽叶和其他部分)

应用

•快速提取高纯度 RNA 适用于所有常规分子生物学程序,包括:
•RT-PCR
• RT-qPCR
• Northern 印迹
•核酸酶保护试验
仅供科研使用。不可用于诊断程序。
规格
洗脱体积50 μL
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于(应用)RT-PCR、qPCR、cDNA 文库构建、NGS、微阵列分析、印迹杂交、Northern 印迹、体外翻译、核酸酶保护测定、核酸标记
高通量能力不兼容高通量应用(手动)
纯化时间20分钟(样本均质后)
数量250 次制备
规格小型
运输条件室温
原始材料量≤100 mg
产量高达 65 μg
分离技术硅胶离心柱
样品类型植物
Unit SizeEach
内容与储存
• 200 mL Plant RNA Lysis Solution; store at room temperature
• 200 mL Wash Buffer WB 1 (concentrated); room temperature
• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Water, Nuclease-free; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Is any GeneJET kit suitable for RNA purification from formalin-fixed paraffin-embedded FFPE tissues?

We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.