β-半乳糖苷酶检测试剂盒
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Invitrogen™

β-半乳糖苷酶检测试剂盒

β-半乳糖苷酶检测试剂盒提供了所需的试剂，可用于快速测定转染了 lacZ 基因表达质粒的细胞中表达的活性 β-半乳糖苷酶水平。lacZ 细菌基因通常用作真核细胞转染实验中的报告基因构建体。lacZ 的基因产物 β-半乳糖苷酶对细胞裂解物中的蛋白水解具有抗性，并且其活性很容易测定。β-半乳糖苷酶催化了解更多信息
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货号数量
K14550180 mL
货号 K145501
价格（CNY)
6,501.00
Each
添加至购物车
数量:
80 mL
价格（CNY)
6,501.00
Each
添加至购物车
β-半乳糖苷酶检测试剂盒提供了所需的试剂，可用于快速测定转染了 lacZ 基因表达质粒的细胞中表达的活性 β-半乳糖苷酶水平。lacZ 细菌基因通常用作真核细胞转染实验中的报告基因构建体。lacZ 的基因产物 β-半乳糖苷酶对细胞裂解物中的蛋白水解具有抗性，并且其活性很容易测定。β-半乳糖苷酶催化 β-半乳糖苷酶的水解，例如邻硝基苯基-D-半乳糖苷 (ONPG)。ONPG 至 ONP 阴离子的水解产生亮黄色，其在 420nm 处峰值吸光度可使用分光光度计进行定量。
10.7 基因表达
仅供科研使用。不可用于诊断程序。
规格
检测β-Gal (lacZ) 检测
产品类型β-半乳糖苷酶测定试剂盒
数量80 mL
底物ONPG
靶标β-半乳糖苷酶
检测方法比色法
Unit SizeEach
内容与储存
β-半乳糖苷酶检测试剂盒含有 10X PBS（磷酸盐缓冲液）、裂解缓冲液、切割缓冲液、β-巯基乙醇、ONPG、终止缓冲液。将 ONPG 储存在 -20°C 条件下。所有其他组分均在室温或 +4°C 条件下储存。如果储存恰当，所有试剂可以确保 6 个月稳定。

常见问题解答 (FAQ)

Are detergents compatible with the β-Gal (ONPG) assay kit (K1455-01)?

Neither NP40 nor Triton X-100 (1%) should interfere with the reaction, although there is a chance that a precipitate may form when the detergent is added to the b-Gal assay reagent.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.