PureLink™ PCR 纯化试剂盒
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
We have updated the storage temperature of the purification columns for this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
PureLink™ PCR 纯化试剂盒
PureLink™ PCR 纯化试剂盒
PureLink™ PCR 纯化试剂盒
Invitrogen™

PureLink™ PCR 纯化试剂盒

无需繁琐的凝胶纯化
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货号数量
K31000150 次制备
K310002250 次制备
货号 K310001
价格（CNY)
1,453.00
飞享价
Ends: 31-Dec-2025
1,851.00
共减 398.00 (22%)
Each
添加至购物车
数量:
50 次制备
价格（CNY)
1,453.00
飞享价
Ends: 31-Dec-2025
1,851.00
共减 398.00 (22%)
Each
添加至购物车
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.

Advantages of the PureLink PCR Purification Kit include:

  • 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
  • No need for tedious sample pH adjustments necessary with other kits
  • Convenient and rapid protocol, completed in less than 10 minutes
  • Optional size-selection buffers included for selectively purifying 300–600 bp fragments

The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.

仅供科研使用。不可用于诊断程序。
规格
产品规格试剂盒
高通量能力兼容高通量应用
分离技术离心柱
产品线PureLink™
产品类型PCR 纯化试剂盒
数量50 次制备
运行时间10 min。
样品类型PCR 产品
运输条件室温
靶标来自酶促反应（例如 PCR）的 DNA
适用于（应用）二代测序（NGS）, 克隆
Unit SizeEach
内容与储存
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.

常见问题解答 (FAQ)

我能否使用PureLink PCR纯化试剂盒来纯化限制性内切酶酶切后的质粒DNA？

是的，您可以使用我们的试剂盒来纯化限制性内切酶酶切后的质粒DNA。

我能否使用PureLink PCR纯化试剂盒来开展凝胶纯化实验？

PureLink PCR纯化试剂盒与PureLink 凝胶纯化试剂盒两者间的缓冲液和操作步骤均不同。因此，您可以换用离心柱，但须注意使用正确的缓冲液和实验方案。

应用PureLink PCR纯化试剂盒（PureLink PCR Clean-Up Kit）可纯化的DNA片段大小范围是多少？

纯化的片段大小介于100 bp与12 kb之间。我们的试剂盒附带两款缓冲液，结合缓冲液（B2）与结合缓冲液HC（B3）。结合缓冲液HC（B3）能够去除引物二聚体和小于300bp的扩增失败的短PCR产物。

Can I use the PureLink PCR Purification Kit to clean up restriction digested plasmid DNA?

Yes, you can use our kit to clean up restriction digested plasmid DNA.

Can I use the PureLink PCR Purification Kit to do a gel purification?

The buffers and procedure are different for the PureLink PCR Purification Kit and PureLink Gel Purification Kit. Therefore, you can swap the column, but the right buffer and protocol must be used.

View all PureLink™ PCR 纯化试剂盒 FAQs