Ni-NTA 纯化系统

引用和文献 (4)

Invitrogen™

Ni-NTA 纯化系统

Ni-NTA 纯化系统设计用于纯化含有多聚组氨酸 (6xHis) 序列的重组蛋白。该试剂盒使用 Ni-NTA 镍螯合树脂，并随附天然和变性缓冲液，用于在不同条件下高效纯化重组蛋白。了解更多信息

货号	数量
K95001	6 purifications

货号 K95001

价格（CNY)

13,014.00

添加至购物车

6 purifications

请求批量或定制报价

价格（CNY)

13,014.00

添加至购物车

Ni-NTA 纯化系统设计用于纯化含有多聚组氨酸 (6xHis) 序列的重组蛋白。该试剂盒使用 Ni-NTA 镍螯合树脂，并随附天然和变性缓冲液，用于在不同条件下高效纯化重组蛋白。

仅供科研使用。不可用于诊断程序。

产品规格悬浮

产品类型Ni-NTA 纯化系统

数量6 purifications

蛋白标记His 标签

Unit SizeEach

内容与储存

六个 2 mL 树脂柱和用于天然和变性纯化的缓冲液。十毫升 Ni-NTA 琼脂糖。在 +4°C 下储存。妥善储存时，所有试剂均可保证稳定储存 6 个月。

常见问题解答 (FAQ)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用和文献 (4)

引用和文献

Abstract

A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.

Authors:Howard PW, Maurer RA

Journal:J Biol Chem

PubMed ID:7673116

'Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription. Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined. Recent reports suggest that phosphorylation of ... More

Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).

Authors: Bhakta S; Bartes A; Bowman K G; Kao W M; Polsky I; Lee J K; Cook B N; Bruehl R E; Rosen S D; Bertozzi C R; Hemmerich S;

Journal:J Biol Chem

PubMed ID:10956661

'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin ... More

Probable Identification of a Membrane-Associated Repressor of Bacillus subtilis DNA Replication as the E2 Subunit of the Pyruvate Dehydrogenase Complex

Authors:Andrew Stein and William Firshein*

Journal:J Bacteriol

PubMed ID:10735853

Two Bacillus subtilis lysogenic libraries were probed by an antibody specific for apreviously described membrane-associated inhibitor of B. subtilis DNA replication(J. Laffan and W. Firshein, Proc. Natl. Acad. Sci. USA 85:7452-7456, 1988). Threeclones that reacted strongly with the antibody contained an entire open reading frame.Sequencing identified one of the clones ... More

Sox9 and p300 cooperatively regulate chromatin-mediated transcription.

Authors:Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H,

Journal:J Biol Chem

PubMed ID:16109717

Chromatin structure is a fundamental component of gene regulation, expression, and cellular differentiation. We have previously reported that the multifunctional coactivator p300 is a member of the Sox9-related transcriptional apparatus and activates Sox9 (Sry-type high-mobility-group box 9)-dependent transcription during chondrogenesis. However, the mechanism of synergy between Sox9 and p300 in ... More