可通过几乎不发出荧光且可透过细胞的钙黄绿素 AM 和高强度荧光钙黄绿素的酶催化转化,确定细胞内普遍存在的酯酶活性,从而区分活细胞。钙黄绿素在活细胞内保存完好,且会在活细胞中产生强烈且均匀的绿色荧光(激发/发射波长为 ∼495/∼515 nm)。使用传统 FITC/GFP 滤光片组可检出钙黄绿素。SYTOX Deep Red 染料可进入细胞膜受损的细胞,与 DNA 结合后荧光增强数倍。死亡或质膜受损的细胞核可发出明亮的荧光,使用 CY5/深红色滤光片组(激发/发射波长最大值:660/682 nm)。
Can LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red)(Cat. No. L32250) be used on bacteria, fungi or protozoa?
We have not validated the use of LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red)(Cat. No. L32250) on bacteria, fungi or protozoa. In general, calcein, AM does not work well with bacteria due to their low intracellular esterase activity. Some protozoa may readily engulf cell impermeant stains and result in intracellular staining of live cells.
What is the expiration date/warranty period for LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red)?
If an expiration date or warranty period is not posted on any public-facing document, the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) (Cat. Nos. L32250) have the standard warranty period of 1 year from the date of shipment.
What is the concentration of the Calcein, AM and SYTOX Deep Red Nucleic Acid stain in the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) (Cat. No. L32250)?
The concentrations of Calcein, AM and SYTOX Deep Red Nucleic Acid stain in the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) (Cat. No. L32250) are proprietary. If you need to use a defined concentration of Calcein, AM we offer this as a standalone product (Cat. Nos. C3099 and C3100MP). If you need to use a defined concentration of SYTOX Deep Red Nucleic Acid stain we offer this as a standalone product (Cat. Nos. S11380 and S11380).