来自人血浆的低密度脂蛋白，BODIPY™ FL 复合物 (BODIPY™ FL LDL)

引用和文献 (19)

Invitrogen™

来自人血浆的低密度脂蛋白，BODIPY™ FL 复合物 (BODIPY™ FL LDL)

人低密度脂蛋白 (LDL) 是大蛋白复合物 (∼500,000 Da)，其可与脊椎动物细胞表面的特定受体结合，通过受体介导的内吞作用传递胆固醇 — 标记的 LDL 复合物是研究这一现象的有用工具。这些实验通常通过加入荧光标记的了解更多信息

货号	数量
L3483	200 μL

货号 L3483

价格（CNY)

8,184.00

添加至购物车

200 μL

价格（CNY)

8,184.00

添加至购物车

人低密度脂蛋白 (LDL) 是大蛋白复合物 (∼500,000 Da)，其可与脊椎动物细胞表面的特定受体结合，通过受体介导的内吞作用传递胆固醇 — 标记的 LDL 复合物是研究这一现象的有用工具。这些实验通常通过加入荧光标记的 LDL 来培养细胞，并通过显微镜或流式细胞术进行分析。或者，荧光标记的 LDL 可注入检测动物，并且可在指定的时间段后分析标记的分布。我们提供一个未标记的 LDL 和两类标记的 LDL：含有未修饰载脂蛋白的 LDL（用于研究正常胆固醇传递和内吞）和含有乙酰化 (Ac) 载脂蛋白的 LDL（用于研究特异性表达这种乙酰化 LDL 的受体的细胞类型（即内皮细胞和小胶质细胞））。

LDL 规格：

•标记 (Ex/Em)：BODIPY™ FL (515/520)
• 乙酰化：否
• 含量：200 µL (1.0 mg/mL)

持续制备新鲜的 LDL
我们大约每两个月采用新鲜人血浆制备 LDL 和 AcLDL 产品。非乙酰化 LDL 产品在其制备后两周内发货。所有的乙酰化 LDL 产品均持续供应。

非乙酰化与乙酰化 LDL
含未修饰载脂蛋白的 LDL 可用于研究正常胆固醇传递和内吞。如果 LDL 的载脂蛋白的赖氨酸残基被乙酰化，则 LDL 复合物不再与 LDL 受体结合，而是由拥有特异性针对该修饰形式的“清道夫”受体的内皮细胞和小胶质细胞摄取。

标记的 LDL 的主要应用
LDL 复合物的其中一些应用包括：
•细胞表面 LDL 受体的计数以及内吞后分析其运动和聚集
• 在成纤维细胞中定量分析 LDL 受体活性（替代放射性标记的 LDL 测定）
• 研究不同细胞系中的 LDL 表达并识别 LDL 受体缺陷

仅供研究使用。不得用于任何动物或人类的治疗或诊断。

仅供科研使用。不可用于诊断程序。

最大浓度1 mg⁄ml

检测方法荧光

染料类型BODIPY 染料

数量200 μL

运输条件湿冰

产品线BODIPY

产品类型补充剂

Unit SizeEach

内容与储存

请避光冷藏 (2–8°C) 储存。

常见问题解答 (FAQ)

Can the LDL in BODIPY FL dye-labeled LDL be oxidized?

We do not recommend doing this as the oxidation process (such as oxidation with copper sulphate) may result in the degradation of the BODIPY FL dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the LDL in BODIPY FL LDL acetylated?

The LDL in BODIPY FL LDL is not acetylated.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use methanol or acetone for fixation of cells stained with BODIPY FL LDL?

If the BODIPY FL LDL is applied to live cells first and then fixed, the use of methanol or acetone fixation is not recommended. Exposure to alcohols, acetone, and other organic solvents can dissociate the BODIPY FL dye from the LDL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Will BODIPY FL LDL be retained upon permeabilization?

No. Exposure to detergents and surfactants can dissociate the BODIPY FL dye from the LDL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the BODIPY FL dye bound to the LDL in BODIPY FL LDL?

The BODIPY FL dye is complexed to hydrophobic domains of the LDL; it is not covalently attached.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (19)

引用和文献

Abstract

LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor.

Authors:Zelcer N, Hong C, Boyadjian R, Tontonoz P,

Journal:Science

PubMed ID:19520913

'Cellular cholesterol levels reflect a balance between uptake, efflux, and endogenous synthesis. Here we show that the sterol-responsive nuclear liver X receptor (LXR) helps maintain cholesterol homeostasis, not only through promotion of cholesterol efflux but also through suppression of low-density lipoprotein (LDL) uptake. LXR inhibits the LDL receptor (LDLR) pathway ... More

Specific binding of human low-density lipoprotein to the surface of schistosomula of Schistosoma mansoni and ingestion by the parasite.

Authors:Bennett MW, Caulfield JP

Journal:Am J Pathol

PubMed ID:2024706

'Low-density lipoproteins (LDL) may be important in human schistosomiasis because LDL bound to the surface of the parasite inhibits the binding of anti-schistosomal antibodies. Low-density lipoproteins also may serve as a source of lipids for the parasite membrane synthesis. Here LDL fluorescently labeled with carbocyanine dye (DiI-LDL) was used to ... More

An actin cytoskeleton with evolutionarily conserved functions in the absence of canonical actin-binding proteins.

Authors:Paredez AR, Assaf ZJ, Sept D, Timofejeva L, Dawson SC, Wang CJ, Cande WZ,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21444821

Giardia intestinalis, a human intestinal parasite and member of what is perhaps the earliest-diverging eukaryotic lineage, contains the most divergent eukaryotic actin identified to date and is the first eukaryote known to lack all canonical actin-binding proteins (ABPs). We sought to investigate the properties and functions of the actin cytoskeleton ... More

Beta2 integrins modulate the initiation and progression of atherosclerosis in low-density lipoprotein receptor knockout mice.

Authors:Merched A, Tollefson K, Chan L,

Journal:Cardiovasc Res

PubMed ID:19843511

Beta2 integrin-mediated adhesion is thought to be a key event in cardiovascular disease. However, results of clinical trials targeting these molecules have been disappointing. Here, we investigated the effect of inactivation of beta2 integrins at different stages of atherosclerosis by timed bone marrow transplantation (BMT) of CD18(-/-) cells in low-density ... More

Cholesterol synthesis and import contribute to protective cholesterol increments in acute myeloid leukemia cells.

Authors:Banker DE, Mayer SJ, Li HY, Willman CL, Appelbaum FR, Zager RA,

Journal:Blood

PubMed ID:15161671

Cholesterol levels are abnormally increased in many acute myeloid leukemia (AML) samples exposed in vitro to chemotherapy. Blocking these acute cholesterol responses selectively sensitizes AML cells to therapeutics. Thus, defining the molecular mechanisms by which AML cells accomplish these protective cholesterol increments might elucidate novel therapeutic targets. We now report ... More

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