LIVE/DEAD™ 精子活力检测试剂盒
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LIVE/DEAD™ 精子活力检测试剂盒
引用和文献 (69)
Invitrogen™

LIVE/DEAD™ 精子活力检测试剂盒

LIVE/DEAD 精子活力检测试剂盒提供了一种新型的荧光检测方法，可用于通过荧光显微镜或流式细胞分析对精子的活力和受精潜力进行分析。可渗透细胞膜的 SYBR® 14 核酸染色剂使用绿色荧光标记活精子，而不可渗透细胞膜的碘化丙啶则使用红色荧光标记细胞膜受损精子的核酸。了解更多信息
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货号数量
L70111 kit
货号 L7011
价格（CNY)
5,904.00
飞享价
Ends: 31-Dec-2025
8,003.00
共减 2,099.00 (26%)
Each
添加至购物车
数量:
1 kit
价格（CNY)
5,904.00
飞享价
Ends: 31-Dec-2025
8,003.00
共减 2,099.00 (26%)
Each
添加至购物车
LIVE/DEAD 精子活力检测试剂盒提供了一种新型的荧光检测方法，可用于通过荧光显微镜或流式细胞分析对精子的活力和受精潜力进行分析。可渗透细胞膜的 SYBR® 14 核酸染色剂使用绿色荧光标记活精子，而不可渗透细胞膜的碘化丙啶则使用红色荧光标记细胞膜受损精子的核酸。
仅供科研使用。不可用于诊断程序。
规格
细胞类型真核细胞、精子细胞
描述LIVE/DEAD™ 精子活力检测试剂盒
检测方法荧光
染料类型其他标记或染料
产品规格管、玻片
数量1 kit
运输条件室温
颜色绿色、红色
发射516、617 nm
适用于（应用）活力测定试剂盒
适用于（设备）荧光显微镜, 流式细胞仪
产品线LIVE/DEAD
产品类型精子活力测定试剂盒
Unit SizeEach
内容与储存
在冷柜（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

我如何为LIVE/DEAD细胞活力实验准备死细胞对照？

有两种简单的方法。一种是通过60°C放置20分钟热灭活细胞。第二种是将细胞放入70%乙醇。乙醇固定的细胞可以在冰箱中无限期储存直到使用，可达好几年。

1.离心细胞、使细胞沉淀并去除上清
2.固定细胞：在15mL含有细胞团的试管中加入10 mL70%冰乙醇，先开始逐滴加入后震荡，混合均匀。
3.在冰箱中储存直到使用。
4.快使用时，水洗两次且在选择的缓冲液中重悬。

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (69)

引用和文献
Abstract
Seminal plasma addition attenuates the dilution effect in bovine sperm.
Authors:Garner DL,Thomas CA,Gravance CG,Marshall CE,DeJarnette JM,Allen CH
Journal:Theriogenology
PubMed ID:11467516
Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More
Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.
Authors:Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV
Journal:Biol Reprod
PubMed ID:11870056
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More
Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).
Authors:Chalah T, Brillard JP
Journal:Theriogenology
PubMed ID:10732141
'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More
Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.
Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M
Journal:Anim Reprod Sci
PubMed ID:12695055
'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
Journal:Cytometry
PubMed ID:10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More
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