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引用和文献 (139)
Invitrogen™
SPQ（6-甲氧基-N-(3-磺丙基)喹啉鎓，内盐）
氯化物指示剂 SPQ 的最大吸收/发射波长为 ∼344/443，并通过扩散限制碰撞淬灭法检测 Cl-。了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂了解更多信息
| 货号 | 数量 |
|---|---|
| M440 | 100 mg |
货号 M440
价格（CNY)
2,531.00
飞享价
Ends: 31-Dec-2025
3,320.00共减 789.00 (24%)
Each
数量:
100 mg
价格（CNY)
2,531.00
飞享价
Ends: 31-Dec-2025
3,320.00共减 789.00 (24%)
Each
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量100 mg
运输条件室温
适用于（设备）共聚焦显微镜, 流式细胞仪
产品类型氯化物指示剂
Unit SizeEach
内容与储存
室温避光储存。
引用和文献 (139)
引用和文献
Abstract
Identification by site-directed mutagenesis of three arginines in uncoupling protein that are essential for nucleotide binding and inhibition.
Journal:J Biol Chem
PubMed ID:9312070
Primary regulation of uncoupling protein is mediated by purine nucleotides, which bind to the protein and allosterically inhibit fatty acid-induced proton transport. To gain increased understanding of nucleotide regulation, we evaluated the role of basic amino acid residues using site-directed mutagenesis. Mutant and wild-type proteins were expressed in yeast, purified,
Fluorescent indicators of ion concentrations.
Journal:Methods Cell Biol
PubMed ID:2538708
Efficient endocytosis of the cystic fibrosis transmembrane conductance regulator requires a tyrosine-based signal.
Journal:J Biol Chem
PubMed ID:9920908
'We previously demonstrated that the cystic fibrosis transmembrane conductance regulator (CFTR) is rapidly endocytosed in epithelial cells (Prince, L. S., Workman, R. B., Jr., and Marchase, R. B. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 5192-5196). To determine the structural features of CFTR required for endocytosis, we prepared
Estimation of intracellular chloride activity in isolated perfused rabbit proximal convoluted tubules using a fluorescent indicator.
Journal:Biophys J
PubMed ID:3395662
'The methodology has been developed to measure cell chloride activity by fluorescence microscopy using the chloride-sensitive dye, 6-methoxy-1-(3-sulfonatopropyl)quinolinium (SPQ). SPQ was loaded into cells of the in vitro microperfused rabbit proximal convoluted tubule by a 10 min luminal perfusion with 20 mM SPQ at 38 degrees C. Fluorescence was excited
Regulation of Cl/HCO3 exchange in gastric parietal cells.
Journal:Cell Regul
PubMed ID:1742343
'Microspectrofluorimetry of the fluorescent indicators 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein and 6-methoxy-N-(3-sulfopropyl)-quinolinium was used to measure intracellular pH (pHi), intracellular Cl (Cli), and transmembrane fluxes of HCO3 and Cl in single parietal cells (PC) in isolated rabbit gastric glands incubated in HCO3/CO2-buffered solutions. Steady-state pHi was 7.2 in both resting (50 microM cimetidine) and