In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins.
Authors:Kaufman BA, Newman SM, Hallberg RL, Slaughter CA, Perlman PS, Butow RA
Journal:Proc Natl Acad Sci U S A
PubMed ID:10869431
The segregating unit of mtDNA is a protein-DNA complex called the nucleoid. In an effort to understand how nucleoid proteins contribute to mtDNA organization and inheritance, we have developed an in organello formaldehyde crosslinking procedure to identify proteins associated with mtDNA. Using highly purified mitochondria, we observed a time-dependent crosslinking ... More