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引用和文献 (52)
Invitrogen™
Quant-iT™ OliGreen™ ssDNA 定量试剂盒和 Quant-iT OliGreen ssDNA 试剂
Quant-iT™ OliGreen ssDNA 定量试剂盒和 Quant-iT OliGreen ssDNA 试剂含有对溶液中的寡核苷酸和单链 DNA (ssDNA) 进行定量的超灵敏绿色荧光核酸染色剂。
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| 货号 | 数量 | 产品类型 |
|---|---|---|
| O11492 | 1 kit | Quant-iT OliGreen ssDNA 试剂盒 |
| O7582 | 1 mL | Quant-iT OliGreen ssDNA 试剂 |
货号 O11492
价格（CNY)
6,263.00
Online Exclusive
Ends: 31-Dec-2025
8,063.00共减 1,800.00 (22%)
Each
数量:
1 kit
产品类型:
Quant-iT OliGreen ssDNA 试剂盒
价格（CNY)
6,263.00
Online Exclusive
Ends: 31-Dec-2025
8,063.00共减 1,800.00 (22%)
Each
使用 Quant-iT OliGreen ssDNA 定量试剂盒和试剂轻松定量 ssDNA。该定量试剂盒和试剂无需高浓度样品即可对单链 DNA 进行灵敏定量。使用该定量试剂盒或 Quant-iT OliGreen ssDNA 试剂可定量低至 100 pg/mL 的样品。
使用 Quant-iT OliGreen ssDNA 测定试剂盒和 Quant-iT OliGreen ssDNA 试剂准确定量 ssDNA 和其他寡核苷酸，无需高浓度样品。Quant-iT OliGreen ssDNA 测定试剂盒含有对溶液中的寡核苷酸和单链 DNA (ssDNA) 进行定量的超灵敏绿色荧光核酸染色剂。Quant-iT™ OliGreen™ ssDNA 测定试剂，让研究人员可以利用荧光酶标仪的激发和发射波长，对低至 1 ng/mL 的寡核苷酸或 ssDNA（200 µL 测定体积中含 200 pg）进行定量。该灵敏度超过吸光度方法的10,000倍。
除染料外，OliGreen ssDNA 测定试剂盒还包括缓冲液和寡核苷酸标准品。OliGreen 试剂也可作为独立的 Quant-iT OliGreen ssDNA 试剂提供。
除染料外，OliGreen ssDNA 测定试剂盒还包括缓冲液和寡核苷酸标准品。OliGreen 试剂也可作为独立的 Quant-iT OliGreen ssDNA 试剂提供。
仅供科研使用。不可用于诊断程序。
规格
激发/发射500/525
适用于（设备）微孔板读数仪
反应次数200次测定（2 mL 测定体积）
产品线OLIGREEN、Quant-iT
产品类型Quant-iT OliGreen ssDNA 试剂盒
定量范围200 pg 至 2 μg
数量1 kit
运输条件室温
检测方法荧光
Unit SizeEach
常见问题解答 (FAQ)
为什么我用Qubit Assay检测时会得到负的荧光值？
我有一个Quant-iT DNA试剂盒，而想在Qubit荧光计上使用。可以吗？
这些Quant-iT试剂盒的适用pH范围是多少？
我试图对一些带有荧光基团标记的DNA进行定量。这可行吗？
DNA长度对dsDNA检测结果有影响吗？
引用和文献 (52)
引用和文献
Abstract
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
Journal:Nucleic Acids Res
PubMed ID:14654700
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of
Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease.
Journal:Gut
PubMed ID:15082587
BACKGROUND AND AIMS: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. PATIENTS AND METHODS: The mucosa associated
Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection.
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:15142831
'Prior studies of intestinal adaptation after massive small bowel resection (SBR) have focused on growth factors and their effects on amplification of the gut mucosa. Because adaptive changes have also been described in intestinal smooth muscle, we sought to determine the effect of targeted smooth muscle growth factor overexpression on
Gene expression in human skeletal muscle: alternative normalization method and effect of repeated biopsies.
Journal:Eur J Appl Physiol
PubMed ID:16151837
'The reverse transcriptase-polymerase chain reaction (RT-PCR) method has lately become widely used to determine transcription and mRNA content in rodent and human muscle samples. However, the common use of endogenous controls for correcting for variance in cDNA between samples is not optimal. Specifically, we investigated (1) a new normalization method
The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells.
Journal:Nucleic Acids Res
PubMed ID:15653633
'Eukaryotic chromatin structure limits the initiation of DNA replication spatially to chromosomal origin zones and temporally to the ordered firing of origins during S phase. Here, we show that the level of histone H4 acetylation correlates with the frequency of replication initiation as measured by the abundance of short nascent