Pacific Blue™ C5-maleimide is an excellent reagent for thiol-selective modification, quantitation and analysis. In this reaction, the thiol is added了解更多信息
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货号
数量
P30506
1 mg
货号 P30506
价格(CNY)
5,340.00
Each
添加至购物车
数量:
1 mg
价格(CNY)
5,340.00
Each
添加至购物车
Pacific Blue™ C5-maleimide is an excellent reagent for thiol-selective modification, quantitation and analysis. In this reaction, the thiol is added across the double bond of the maleimide to yield a thioether. This compound does not react with methionine, histidine or tyrosine. Reaction of Pacific Blue™ C5-maleimide with amines usually requires a higher pH than reaction of maleimides with thiols.
A robust method for production of MHC tetramers with small molecule fluorophores.
Authors:Ramachandiran V, Grigoriev V, Lan L, Ravkov E, Mertens SA, Altman JD
Journal:J Immunol Methods
PubMed ID:17187819
Tetramers of major histocompatibility complex molecules (MHC) are now well-established reagents for the detection of antigen-specific T cells by flow cytometry. MHC tetramers are prepared by mixing enzymatically biotinylated MHC molecules with commercial preparations of streptavidin, usually conjugated to a fluorescent phycobiliprotein such as phycoerythrin (PE) or allophycocyanin (APC). While ... More