pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
Invitrogen™

pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒

pHrodo Deep Red 哺乳动物和细菌细胞标记试剂盒提供了您在吞噬作用和胞吞作用研究中轻松标记活细胞(哺乳动物或细菌)所需的试剂。pHrodo Deep Red 染料仅在暴露于酸性环境时才发出荧光,因此针对区分吞噬细胞和非吞噬细胞进行了优化了解更多信息
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货号数量
P353571 个试剂盒
货号 P35357
价格(CNY)
4,941.00
Online Exclusive
Ends: 31-Dec-2026
6,862.00
共减 1,921.00 (28%)
Each
添加至购物车
数量:
1 个试剂盒
价格(CNY)
4,941.00
Online Exclusive
Ends: 31-Dec-2026
6,862.00
共减 1,921.00 (28%)
Each
添加至购物车
pHrodo Deep Red 哺乳动物和细菌细胞标记试剂盒提供了您在吞噬作用和胞吞作用研究中轻松标记活细胞(哺乳动物或细菌)所需的试剂。pHrodo Deep Red 染料仅在暴露于酸性环境时才发出荧光,因此针对区分吞噬细胞和非吞噬细胞进行了优化。低背景 pHrodo Deep Red 标记试剂盒使研究胞吞作用和吞噬作用比以往更容易,结果确定性更高且优化步骤更少,因为 pHrodo Deep Red 染料仅在晚期内体和溶酶体中发荧光。

pHrodo Deep Red 哺乳动物和细菌细胞标记试剂盒的特点包括:
多通路—Cy5 通道可进行多色实验
简单—易于遵循的分步方案
出色的信号窗口—中性 pH 值条件下背景极低,能够实现极大信噪比以提高结果确定性
低 pKa—可消除来自非内化物质的非特异性信号,并且仅在晚期内体和溶酶体中发荧光

pHrodo Deep Red 是一种低背景 pH 传感器染料,其在中性条件下不显示信号,并且仅在酸性环境中发荧光。这种独特的特性有助于实现快速检测开发,并提高抗体内吞和吞噬途径研究结果的确定性。pHrodo Deep Red 染料有助于更好地辨别细胞外的内化物质,因为其 pKa 约为 5,在进入晚期内体和溶酶体之后才发荧光。pHrodo Deep Red 染料可使用 Cy5 荧光过滤器套件检测到,并经过验证可用于多种应用,包括流式细胞分析、荧光显微镜检测和高内涵分析 (HCA)。

了解更多关于 pHrodo 染料的信息›

仅供科研使用。不可用于人或动物的治疗或诊断。
规格
描述pHrodo™ Deep Red 哺乳动物和细菌细胞标记试剂盒
适用于(应用)细胞分析
适用于(设备)流式细胞仪
产品线pHrodo
产品类型细胞标记试剂盒
数量1 个试剂盒
Unit SizeEach
内容与储存
在 –20°C 干燥条件下避光储存。

常见问题解答 (FAQ)

May I use other fluorophores with cells labeled with pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357)?

Yes. The pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357) will label the surface of the cells. You may use other fluorophores to stain or label other organelles in fluorescent colors that do not overlap with the far-red signal of pHrodo Deep Red.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long will the cells remain viable after labeling with the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357)?

How long cells remain viable after labeling with the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357) is unknown. Viability after labeling is dependent upon the inherent properties of the cell and other physiological events that occur while culturing. Assuming the cell type remains viable after labeling, the signal will be halved with each generation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of reactive chemistry/reactive group is utilized by the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357)?

An amine-reactive pHrodo Deep Red TFP ester is used for the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357). The same reactive pHrodo Deep Red TFP Ester is available as a standalone product (Cat. Nos. P35358, P35359), but they are not of the same amount provided in the kit. The amount of reactive dye provided in the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357) is proprietary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.