ProLong™ Live Antifade Reagent, for live cell imaging
ProLong™ Live Antifade Reagent, for live cell imaging
ProLong™ Live Antifade Reagent, for live cell imaging
ProLong™ Live Antifade Reagent, for live cell imaging
Invitrogen™

ProLong™ Live Antifade Reagent, for live cell imaging

ProLong Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging through use了解更多信息
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货号数量
P369745 x 2 mL
P369752 mL
货号 P36974
价格(CNY)
1,730.00
飞享价
Ends: 31-Dec-2025
2,327.00
共减 597.00 (26%)
Each
添加至购物车
数量:
5 x 2 mL
价格(CNY)
1,730.00
飞享价
Ends: 31-Dec-2025
2,327.00
共减 597.00 (26%)
Each
添加至购物车

ProLong Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging through use of enzymes from the plasma membrane of E. coli. These enzymes metabolize the elements that can cause photobleaching. When dyes are illuminated, they can degrade which reduces the sample’s fluorescent intensity and leads to the creation of free radical singlet oxygen which degrades neighboring dye molecules. ProLong Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours. ProLong Live reagent provides photobleach protection in live cell experiments for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker™, LysoTracker™, and CellTracker™dyes. ProLong Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.

仅供科研使用。不可用于诊断程序。
规格
数量5 x 2 mL
运输条件经批准可在室温下或置于湿冰上运输
产品线ProLong™
产品类型Live 抗荧光淬灭试剂
试剂类型抗荧光淬灭溶液
容积(公制)1 mL
Unit SizeEach
内容与储存
5 瓶,每瓶 1 mL。足够用于5000次测定(0.1 mL 成像溶液/测定)或1000次测定(0.5 mL 成像溶液/测定)。
在 ≤-20°C 下储存时,产品至少可稳定保存6个月,至多4个冻融循环。
在 2°C 至 8°C 下储存时,产品应在30天内使用(在这种温度下可能形成沉淀物)。

常见问题解答 (FAQ)

我希望采用抗淬灭封固剂来封固自己的染料标记细胞,防止染料发生光漂白。你们推荐采用哪种封固剂?

染料需要发光成像,在此过程中它们会淬灭或“光漂白”,导致荧光不断变暗、检测效率不断降低。抗淬灭封固剂可以大幅减少光漂白现象。如果您想标记活细胞,可使用ProLong Live抗淬灭试剂。如果您想标记后立即封固细胞,然后立即成像并丢弃样品,可采用SlowFade Diamond 抗淬灭封固剂,因为其可以保持液态并有良好的折射率。如果您想封固样品后存档样品,ProLong Diamond 抗淬灭封固剂会固化以获得更好的折射率,且可存档样品长达数周乃至数月。与其他抗淬灭封固剂不同,此类产品非常适合对荧光蛋白质进行即时成像(无法进行荧光蛋白质存档),并且有含或不含DAPI两种包装规格。更多信息请见此处。

当我拍照时我发现荧光染料的信号淬灭了,我该如何终止这一现象?

当它们暴露在吸收波长强光下时,所有的荧光染料都会在一定程度上出现褪色或“光漂白”现象。这里列有一些光漂白的原因以及解决方案:

1)光漂白的原因:自由基和单线态氧的产生
可能的解决方案:使用具有抗氧化剂和自由基清除剂的抗褪色剂:

i. 对于荧光染料和蛋白质的活细胞成像,我们建议使用ProLong Live Antifade Reagent,该试剂可以加入到细胞培养基或缓冲液中。ProLong Live Antifade Reagent可以在24小时内不影响细胞的健康的前提下显著增加试剂和荧光蛋白,如GFP的稳定性。
ii. 对于直接分析与短期储存的固定样品,我们建议使用SlowFade Diamond Antifade Mountant(该试剂保持液态并能即看即用,然后在一天内丢弃样品)。
iii. 对于长期分析Alexa Fluor染料的固定样品,我们推荐固化的封固剂如ProLong Diamond Antifade Mountant(其可减慢自由基运动)。
iv. -对于长期分析所有染料和荧光蛋白的固定样品,我们推荐ProLong Diamond Antifade Mountant(固化后可以保存载玻片)。

2) 光漂白的原因:染料对褪色尤为敏感
可能的解决方案:
i. 选择更耐光染料,比如我们的Alexa Fluor染料
ii.您可以使用复染剂选择和设置像场,然后切换到目标染色加以成像。

3) 光漂白的原因:强光照射
可能的解决方案:
i. 减少曝光,例如降低激光功率或使用中性密度滤光片。
ii. 观察标记样品的时间尽可能的短,并在不观看时关闭遮板。
iii. 使用具有较低数值孔径的物镜,如低功耗物镜。

这里(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades.html?icid=fr-antifade-main)是一个很好的抗淬灭剂选择指南。

After using ProLong Live Antifade Reagent on live samples and then fixing and permeabilizing, may the samples be mounted in ProLong Gold or Diamond Anifade Mountant?

Yes, this is possible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After using ProLong Live Antifade Reagent on my live sample, may I fix and permeabilize the sample?

Yes. We recommend washing off the ProLong Live Antifade Reagent with pre-warmed buffer or media and proceeding to your standard fixation and permeabilization steps.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May I use ProLong Live Antifade Reagent on a fixed/permeabilized sample?

This is possible for immediate viewing, but the sample cannot be archived with ProLong Live Antifade Reagent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.