电穿孔比色皿，0.2 cm

Electroporation uses short, high-voltage electric pulses to overcome the cell membrane and introduce DNA directly into cells. These cuvettes are了解更多信息

货号	数量
P45050	50 each

货号 P45050

价格（CNY)

2,376.00

添加至购物车

50 each

价格（CNY)

2,376.00

添加至购物车

Electroporation uses short, high-voltage electric pulses to overcome the cell membrane and introduce DNA directly into cells. These cuvettes are specifically designed help achieve high DNA transformation efficiency during the introduction of DNA into bacterial, yeast, fungal, and mammalian cells.

Our electroporation cuvettes are precision manufactured from the highest quality materials to ensure optimal and reproducible transformation results.

Electroporation cuvette features
• Compatible with virtually all common electroporation devices
• Gamma-irradiated and individually packaged to ensure sterility
• Color-coded snap cap for easy identification of gap size (blue cap for 0.2 cm cuvette)

Note: Electroporation cuvettes are recommended for use with all of our electroporation competent cells.

仅供科研使用。不可用于诊断程序。

直径（公制）0.2 cm

适用于（设备）电穿孔设备

产品类型电穿孔比色皿

数量50 each

运输条件室温

Unit SizeEach

内容与储存

每袋含有 50 个独立包装的无菌比色皿。室温下储存。

常见问题解答 (FAQ)

我如何才能避免在电转<i>E. coli</i>时出现电火花？

当使用高电压在导电缓冲液中进行电转时，可能会出现电火花。MgCl2和PO4的导电能力尤其强。

以下为一些建议：
1.将克隆反应的离子强度保持在最小。如果反应缓冲液含有高盐，在电转前请将DNA样本用水或TE缓冲液稀释。
2.尽量避免加入导电离子。DNA溶液的体积不应超过反应总体积的5%。例如：40 μl细胞中加入2 μl DNA。
3.确保没有气泡存在。
4.确保电极接触是干净紧密的。擦除电转杯外面的任何沉淀物。
5.要获得最佳结果，细胞应当被加入缝隙的最底部。轻弹电转杯使细胞沉降到底部。

Will Invitrogen cuvettes fit my electroporator?

Our cuvettes are known as the "Potter-style" cuvettes, and they fit most electroporators that accept a standard size cuvette.

How can I avoid arcing during electroporation of E. coli?

When electroporating cells at high voltage in conductive buffers, arcing may occur. MgCl₂ and PO₄ in particular are very conductive.

Some suggestions:

1) Keep ionic strength of cloning reactions to a minimum. If reaction buffers contain high salt, dilute DNA sample in water or TE buffer before electroporation.

2) Minimize addition of conductive ions. The volume of DNA solution should not exceed 5% of the total reaction. Example: 2 ?l DNA per 40 ?l of cells.

3) Be sure no air bubbles are present.

4) Make sure the electrical contacts are clean and tight. Wipe away any condensation on the outside of the cuvette.

5) For best results, the cells should be aliquoted into the bottom of the gap - tap the cuvette gently to help the cells settle to the bottom.

What is meant by "field strength" (kV/cm) when determining settings for electroporation? What are typical settings for different cell types?

The two most important electrical parameters for consideration in electroporation are pulse length and field strength. Field strength is defined as volts/centimeter, where V is equal to the initial peak voltage and cm is equal to the measurement of the gap between the electrodes of the cuvette in centimeters. For example, during electroporation of bacteria at 1,500 Volts in a 0.1 cm cuvette, the field strength would be 15,000 volts/cm, or 15 kV/cm. Typically, electroporation of bacteria requires field strengths of greater than 15 kV/cm. Yeast cells require 6-8 kV/cm, and mammalian cells often require optimization between 0.5 to 2.5 kV/cm.

What are recommended settings for bacterial electroporation of 80 ul cells in 0.2 cm cuvettes? What is the maximum cell volume to use in a 0.2 cm cuvette?

Depending upon the manufacturer of the electroporator, the settings may range in value (25 -50 ?F, 100-200 ohms, 1500-2500 volts). Follow the manufacturer's recommended settings for bacterial electroporation. The maximum recommended cell volume that should be used for a 0.2 cm cuvette without compromising efficiency is 160 ?l.