Power Blotter 标准卡匣

当Thermo Scientific Power Blotter与Towbin转膜缓冲液结合使用时，可在45–60分钟内同时转印1-4块小型凝胶或1-2块中型凝胶。请注意，同时转印的凝胶必须配方相同。

当Thermo Scientific Power Blotter与Thermo Scientific一步转膜缓冲液结合使用时，可在5-10分钟内同时转印1-4块小型凝胶或1-2块中型凝胶。请注意，同时转印的凝胶必须配方相同。

Thermo Scientific Power Blotter（货号22834）由带有激活版印迹软件的Thermo Scientific Power Station（货号22838）和Thermo Scientific Power Blot Cassette（货号22835）组成。该系统专用于将蛋白质从聚丙烯酰胺凝胶上快速半干转印到硝化纤维素膜或PVDF膜上。传统的免疫印迹分析技术需要转印1小时至过夜，才能获得良好的转印结果。与Thermo Scientific一步转膜缓冲液结合使用时，Thermo Scientific Power Blotter只需5-10分钟即可带来等于或优于传统转印技术的转印效率，并且无需预平衡凝胶。这种蛋白质转印时间的显著缩短，是通过优化转膜缓冲液的离子强度和增加通过转印膜组的电流[amps (A)/cm2]实现的。该系统已经过验证，对常用的预制胶和自制SDS-PAGE凝胶均适用。Thermo Scientific Power Blotter也可与Towbin缓冲液一起用于标准半干转实验方案。

The Power Blotter System (Cat. No. PB0012) comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter Cassette (Cat. No. PB0002). The Power Blotter System can transfer one midi or up to two mini gels at the same time. The Power Blotter XL System (Cat. No. PB0013) on the other hand comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter XL Cassette (Cat. No. PB0003). The Power Blotter XL System can transfer up to two midi or four mini gels at the same time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Even though the Power Blotter System/Power Blotter XL System does not have the staining program, this can be introduced. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.