Power Blotter 1-Step™ 转膜缓冲液 (5X)
Power Blotter 1-Step™ 转膜缓冲液 (5X)
Power Blotter 1-Step™ 转膜缓冲液 (5X)
Power Blotter 1-Step™ 转膜缓冲液 (5X)
Invitrogen™

Power Blotter 1-Step™ 转膜缓冲液 (5X)

Invitrogen Power Blotter 1-Step 转印缓冲液设计用于利用 Power Blotter 系统将蛋白从聚丙烯酰胺凝胶 (SDS-PAGE) 快速半干转印至硝酸纤维素膜或了解更多信息
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货号数量
PB7100250 mL
PB73001 L
PB7300X22 x 1 L
货号 PB7100
价格(CNY)
669.00
Each
添加至购物车
数量:
250 mL
价格(CNY)
669.00
Each
添加至购物车
Invitrogen Power Blotter 1-Step 转印缓冲液设计用于利用 Power Blotter 系统将蛋白从聚丙烯酰胺凝胶 (SDS-PAGE) 快速半干转印至硝酸纤维素膜或 PVDF 膜上。该转印缓冲液与常用的检测方法兼容,例如染色、化学发光或荧光检测。

Power Blotter 1-Step 转印缓冲液的特点:
快速—配合兼容的快速半干转系统使用时,其高离子强度配方可在 5 至 12 分钟内完成蛋白转印
经过优化—设计用于 Power Blotter 系统实现无缝和可靠的性能
兼容性强—与其他配备合适的大电流电源的半干转印设备兼容
经济—无需其他快速半干转印设备必备的特殊或昂贵耗材

了解有关半干转印设备的更多信息:Power Blotter 系统

配合 Power Blotter 系统使用时,该缓冲液可在 5 至 12 分钟内完成从凝胶到膜的高效蛋白转印,效率与传统的 Western 印迹技术相当。快速印迹方法需要使用大电流电源(例如 Power Blotter 系统)和优化的高离子强度转印缓冲液(例如 Power Blotter 1-Step 转印缓冲液)。

可通过增加电流,以比传统方法更短的时间获得出色的转印效率。根据转印膜组的表面积保持电流恒定 (∼22–23 mA/cm2),电压不高于 25 V。

与合适的大电流电源匹配时,Power Blotter 1-Step 转印缓冲液可与其他蛋白半干转印设备兼容。此类设备可提供恒定的高电流(1.3至5.0安),以通过转膜缓冲液提供的高离子强度条件快速转印蛋白质。

相关产品
Power Blotter 预切膜和滤纸,硝酸纤维素膜,常规尺寸
Power Blotter 预切膜和滤纸,硝酸纤维素,微型
Power Blotter 预切膜和滤纸,PVDF,常规尺寸
Power Blotter 预切膜和滤纸,PVDF,微型

仅供科研使用。不可用于诊断程序。
规格
数量250 mL
运输条件室温
适用于(应用)蛋白免疫印迹
膜兼容性硝酸纤维素、PVDF
传送模式半干式
产品线1 步法™
类型转印缓冲液
Unit SizeEach

常见问题解答 (FAQ)

What is the difference between the Power Blotter System and the Power Blotter XL System?

The Power Blotter System (Cat. No. PB0012) comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter Cassette (Cat. No. PB0002). The Power Blotter System can transfer one midi or up to two mini gels at the same time. The Power Blotter XL System (Cat. No. PB0013) on the other hand comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter XL Cassette (Cat. No. PB0003). The Power Blotter XL System can transfer up to two midi or four mini gels at the same time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is it possible to do gel staining with the Power Blotter System/Power Blotter XL System?

Even though the Power Blotter System/Power Blotter XL System does not have the staining program, this can be introduced. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the material used for the anode and cathode plates of the Power Blotter Cassette/Power Blotter Cassette XL?

The top (cathode) is stainless steel and the bottom (anode) is platinum-coated stainless steel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The central European or UK power cord for the Power Blotter System/Power Blotter XL System does not work in Switzerland. Do you offer a Swiss power cord?

Yes, you may purchase the Universal Cable Kit (Cat. No. 4377117).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I have lost the power cord for the Power Blotter Station. Is it sold as a stand-alone item?

Here are the SKUs for the power cord. Please order the one appropriate for your region:

- Cat. No. 84858: Power Cord with C13 Connector, North America
- Cat. No. 84859: Power Cord with C13 Connector, Continental Europe and South Korea
- Cat. No. 84860: Power Cord with C13 Connector, United Kingdom and Singapore
- Cat. No. 84855: Chinese 10 Amp Cordset w/ C13 Connector
- Cat. No. 84856: Japanese 15 Amp Cordset w/ C13 Connector
- Cat. No. 84857: Australian 10 Amp Cordset w/ C13 Connector

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.