Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Invitrogen™

Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂

使用荧光 Quant-iT RNA 试剂和测定试剂盒可实现对高丰度 RNA 样品的高选择性和准确定量。相对于双链 DNA,这些试剂盒对 RNA 具有高度选择性,其提供的材料足够以96孔微孔板规格进行至少2000次测定。
Have Questions?
更改视图buttonViewtableView
货号数量检测定量范围
Q328841 mLRNA 定量,高灵敏度5 至 100 ng
Q331401 kitRNA 定量5 至 100 ng
Q102131,000 assaysRNA 定量,宽范围20 至 1000 ng
Q332251000 assaysRNA 定量,扩展范围200 至 10000 ng
货号 Q32884
价格(CNY)
5,786.00
Each
添加至购物车
数量:
1 mL
检测:
RNA 定量,高灵敏度
定量范围:
5 至 100 ng
价格(CNY)
5,786.00
Each
添加至购物车
使用 Quant-iT RNA 定量试剂盒可以实现简单、准确的 RNA 定量,该试剂盒具有宽范围 (BR) 和扩展范围 (XR) 配置且含有独立的高灵敏度 (HS) 试剂。相较于 dsDNA,这些 Quant-iT RNA 定量试剂盒对 RNA 具有高度选择性,可用于实现基于荧光的线性 RNA 检测范围。
Quant-iT RNA 试剂和测定试剂盒
Quant-iT RNA 试剂和测定试剂盒使 RNA 定量分析变得轻松、准确。该试剂盒提供浓缩的检测试剂、稀释缓冲液以及预稀释的 RNA 标准品。相较于双链 DNA,此测定试剂盒对 RNA 具有高度选择性,并提供 5-100 ng RNA 的动态范围。

Quant-iT RNA BR(宽范围)测定试剂盒
Quant-iT RNA BR(宽范围)测定试剂盒提供了一种准确且具有选择性的高丰度 RNA 样品定量方法。原装 Quant-iT RNA 试剂盒具有很高的灵敏度,而 Quant-iT RNA 宽范围试剂盒适用于 RNA 浓度较高的微阵列样品。相较于双链 DNA,此测定试剂盒对 RNA 具有高度选择性,并提供 20-1000 ng RNA 的动态范围。

Quant-iT RNA XR(扩展范围)测定试剂盒
Quant-iT RNA XR(扩展范围)测定试剂盒提供了一种准确且具有选择性的高丰度 RNA 样品定量方法。该检测试剂对于 RNA 具有高度选择性,不能定量 DNA、蛋白或游离核苷酸。该测定试剂盒设计用于准确检测初始浓度为 10 ng/µL 至 10,000 ng/µL 的 RNA 样品(取决于样品体积),线性检测范围为 200–10,000 ng。

每次测定,只需使用提供的缓冲液对试剂进行稀释,加入样品(1 µL 至 20 µL 的任意体积均可接受),然后就可使用基于荧光的酶标仪读取浓度值。对常见污染物,如盐、游离核苷酸、溶剂、洗涤剂和蛋白质等,有着很好的耐受性。

仅供科研使用。不可用于诊断程序。
规格
检测RNA 定量,高灵敏度
激发/发射644/673
适用于(设备)荧光计、微孔板读数仪
反应次数2000 次检测
产品线Quant-iT
定量范围5 至 100 ng
数量1 mL
运输条件室温
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

为什么我用Qubit Assay检测时会得到负的荧光值?

负的荧光值物理上是不可能的。它是由于软件自动扣除背景信号而造成的假象。这意味着你的荧光计检测到背景信号并将其扣除从而牺牲了真实数据。务必做一个仅有缓冲液的对照并评估信号的类型。你可能需要换用另外一块板。

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

引用和文献 (5)

引用和文献
Abstract
Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3-E1 cells through microRNA-153 by targeting Runt-related transcription factor 2.
Authors:Huang Z, Cheng C, Wang J, Liu X, Wei H, Han Y, Yang S, Wang X
Journal:Exp Ther Med
PubMed ID:29904399
'Osteoporosis has become one of the most serious public health problems. Icariin, miR-153 and Runt-related transcription factor 2 (Runx2) have been demonstrated to regulate cell proliferation and differentiation in multiple cells. The aim of the present experiments was to investigate the potential mechanism underlying osteoblast differentiation and cell proliferation of ... More
Transcriptional Characterization Of The Tumor Immune Microenvironment And Its Prognostic Value For Locally Advanced Lung Adenocarcinoma In A Chinese Population.
Authors:Chen Y, Chen H, Mao B, Zhou Y, Shi X, Tang L, Jiang H, Wang G, Zhuang W
Journal:Cancer Manag Res
PubMed ID:31802941
'We investigated the relationship of the transcriptional tumor immune microenvironment with prognosis of patients with locally advanced lung adenocarcinoma (LUAD).'
Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells.
Authors:Elsemüller AK, Tomalla V, Gärtner U, Troidl K, Jeratsch S, Graumann J, Baal N, Hackstein H, Lasch M, Deindl E, Preissner KT, Fischer S
Journal:FASEB J
PubMed ID:30702929
Tissue-resident mast cells (MCs) are well known for their role in inflammatory responses and allergic and anaphylactic reactions, but they also contribute to processes of arterial remodeling. Although ribosomes and cytosolic RNAs are located around secretory granules in mature MCs, their functional role in MC responses remains unexplored. Previous studies ... More
A novel long noncoding RNA FAF inhibits apoptosis via upregulating FGF9 through PI3K/AKT signaling pathway in ischemia-hypoxia cardiomyocytes.
Authors:Shi HJ, Wang MW, Sun JT, Wang H, Li YF, Chen BR, Fan Y, Wang SB, Wang ZM, Wang QM, Wang LS
Journal:J Cell Physiol
PubMed ID:31093967
Long noncoding RNAs (lncRNAs) have been increasingly considered to play an important role in the pathological process of various cardiovascular diseases, which often bind to the proximal promoters of the protein-coding gene to regulate the protein expression. However, the functions and mechanisms of lncRNAs in cardiomyocytes have not been fully ... More
Circulating RNA Transcriptome of Pregnant Women with TSH Just Above the Trimester-Specific Reference and its Correlation with the Hypertensive Phenotype.
Authors:Hirata AHL, Rocha LAJ, da Silva VA, de Almeida RJ, Bacigalupo LDS, Varela P, Martins L, Pesquero JB, Dellê H, Camacho CP
Journal:Sci Rep
PubMed ID:32296081
During gestation, a woman's body undergoes physiological changes that alter thyroid function. Pregnant women with hypothyroidism may exhibit gestational complications, including hypertension and preeclampsia. We investigated differentially expressed genes (DEGs) in circulating RNAs from pregnant women with TSH levels just above the normal range to determine the impact of a ... More