QuantiGene™ Plex 检测试剂盒
QuantiGene™ Plex 检测试剂盒
Invitrogen™

QuantiGene™ Plex 检测试剂盒

QuantiGene Plex 检测试剂盒含有在 96 孔板中进行检测所需的通用试剂、缓冲液和平板,必须与靶标特异性 QuantiGene Plex 检测板(单独出售了解更多信息
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货号数量
QP101450 Plex,3 x 96 孔板
QP101350 Plex,96孔板
QP101510块板
货号 QP1014
价格(CNY)
28,598.00
Each
添加至购物车
数量:
50 Plex,3 x 96 孔板
价格(CNY)
28,598.00
Each
添加至购物车
QuantiGene Plex 检测试剂盒含有在 96 孔板中进行检测所需的通用试剂、缓冲液和平板,必须与靶标特异性 QuantiGene Plex 检测板(单独出售)配合使用。参见相关样品制备试剂和 RNA 对照品

QuantiGene Plex 检测是一种基因表达检测,可对多达 80 个 RNA 转录本靶标进行多通路测量。本检测使用靶标特异性探针捕获目标 RNA,然后采用分支 DNA 技术放大荧光信号,用 Luminex xMAP 仪器读取荧光信号。

困难样品类型—支持 FFPE 组织和血液中降解和交联的 RNA
真正多通路分析—可在同一孔中测量多达 80 个目标基因和看家基因,无交叉反应性
标化平台—使用 96 或 384 孔板规格与 Luminex 200、MagPix 和 FlexMap3D 系统兼容
简单工作流程—采用类似 ELISA 的工作流程可将转录物直接与微珠杂交以及转录本标记
较大的经验证基因库存—可从超过 15,000 个基因中选择创建通路和疾病检测板
快速周转定制检测板—在没有您的基因的情况下,我们可在两周内创建定制检测板

技术
QuantiGene 检测采用已在 VERSANT™ 诊断检测 HIV-1、HCV 和 HBV 中使用数十年的分支 DNA (bDNA) 技术。首先,将细胞裂解或将组织样品均质化以释放靶标 RNA。其次,将寡核苷酸探针组与靶标 RNA 和 Luminex xMAP 微珠组一起孵育过夜。在该孵育过程中,探针与靶标协同杂交,捕获与每个微珠结合的探针,捕获靶 RNA。第三,通过将 bDNA 预扩增剂、扩增剂和标记探针分子依次与靶标杂交进行信号放大。添加链霉亲和素藻红蛋白产生的发光信号与样品中靶标 mRNA 的量成正比。

应用
QuantiGene Plex 检测应用包括候选药物的化合物筛查、生物标志物验证、siRNA 敲低效率、临床样品的前瞻性和/或回顾性分析、miRNA 表达谱、微阵列验证、预测毒理学以及易位和融合基因检测。

仅供科研使用。不可用于诊断程序。
规格
适用于(应用)QuantiGene Plex 384 孔检测
适用于(设备)Luminex instrument
产品线QuantiGene™
样品类型RNA
运输条件蓝冰
检测方法Fluorescence
产品规格96-well
产品类型Plex 测定试剂盒
数量50 Plex,3 x 96 孔板
种属Universal
Unit SizeEach
内容与储存

• 105 μL Proteinase K, –20°C
• 810 μL Blocking Reagent, –20°C
• 34.5 mL Label Probe Solution, 2–8°C
• 34.5 mL Pre-Amplifier Solution, 2–8°C
• 34.5 mL Amplifier Solution, 2–8°C
• 34.5 mL SAPE Solution, 2–8°C
• 36 mL Lysis Mixture, 15–30°C
• 2.4 mL Wash Buffer Component 1, 15–30°C
• 39 mL Wash Buffer Component 2, 15–30°C
• 34.5 mL10x SAPE Wash Buffer Concentrate, 15–30°C
• 3 Magnetic Separation Plates, 15–30°C
• 30 Plate Seals, 15–30°C
• 3 Hybridization Plates, 15–30°C
• 6 Pressure Seals, 15–30°C

Note: Prepared 1x Wash Buffers can be stored for 1 week protected from light at 4°C.

常见问题解答 (FAQ)

Can samples in TRIzol solution be run in the QuantiGene Plex assay?

No, samples in TRIzol solution cannot be run in the QuantiGene Plex assay. However, RNA purified from these samples can be run in the QuantiGene Plex assay.

Can I use the QuantiGene Plex assay with bacteria?

Although the kits were not originally validated for bacteria, we have guidelines for preparing bacterial samples for QuantiGene Plex assays: http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf

Do you offer QuantiGene Plex assay custom services where I can send my samples for you to process?

Yes, please reach out to your Technical Sales Specialist for more information or fill out this form: https://www.thermofisher.com/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html

Can I detect genomic DNA using the QuantiGene Plex assay?

Under the hybridization conditions that are used for the QuantiGene Plex assay, genomic DNA remains double-stranded. Therefore, it is not possible for the probes to bind to DNA. The signal from the probe sets comes only from RNA.

Can I use other lysis buffers with QuantiGene Plex assay samples?

We only recommend the use of QuantiGene Lysis Mixture for preparing cell and blood lysates. The Lysis Mixture has been optimized to provide stability of RNA in the lysates. In addition, QuantiGene Lysis Mixture provides the appropriate buffer conditions for the overnight hybridization.