QuantiGene™ Plex 384 孔检测试剂盒
QuantiGene™ Plex 384 孔检测试剂盒
QuantiGene™ Plex 384 孔检测试剂盒
QuantiGene™ Plex 384 孔检测试剂盒
QuantiGene™ Plex 384 孔检测试剂盒
QuantiGene™ Plex 384 孔检测试剂盒
Invitrogen™

QuantiGene™ Plex 384 孔检测试剂盒

QuantiGene Plex 384 孔测定试剂盒包含在 384 孔板中测定所需的通用试剂、缓冲液和孔板,必须与靶标特异性的 QuantiGene Plex 检测组合了解更多信息
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货号数量
QP10162块板
货号 QP1016
价格(CNY)
60,385.00
Each
添加至购物车
数量:
2块板
价格(CNY)
60,385.00
Each
添加至购物车
QuantiGene Plex 384 孔测定试剂盒包含在 384 孔板中测定所需的通用试剂、缓冲液和孔板,必须与靶标特异性的 QuantiGene Plex 检测组合(单独出售)联用。参见相关样品制备试剂和 RNA 对照品›。QuantiGene Plex 测定试剂盒是一种基因表达测定试剂盒,可对多达80个 RNA 转录本靶标进行多通路测量。本检测使用靶标特异性探针捕获目标 RNA,然后采用分支 DNA 技术放大荧光信号,再用 Luminex xMAP 仪器 (FlexMap3D) 读取荧光信号。QuantiGene Plex 384 孔检测试剂盒的特点包括:
能适应难处理的样品类型—可处理 FFPE 组织和血液中的降解和交联 RNA
真正的多通路分析—可在同一孔中测量多达80个目标基因和管家基因,无交叉反应性
标准化平台—使用与 Luminex FlexMap3D 系统兼容的384孔板规格
简单的工作流程—采用类似 ELISA 的工作流程,直接将转录本与微珠杂交以及进行转录本标记
较大的经验证基因库—可从超过22,000个基因中进行选择,以创建通路和以疾病为主题的检测组合
定制检测组合周转时间短—定制检测组合在3周内即可创建完成技术
QuantiGene 测定试剂盒利用了分支 DNA (bDNA) 技术,这种技术在生物学的各种应用和领域中已使用数十年。首先,将细胞裂解或将组织样品均质化以释放靶标 RNA。其次,将寡核苷酸探针组与靶标 RNA 和 Luminex xMAP 微珠组一起孵育过夜。在该孵育过程中,探针与靶标协同杂交,捕获与每个微珠结合的探针,捕获靶 RNA。第三,通过将 bDNA 预扩增剂、扩增剂和标记探针分子依次与靶标杂交进行信号放大。加入链霉素亲和素藻红蛋白会产生与样品中存在的靶标 mRNA 数量成正比的发光信号。应用
QuantiGene Plex 384 孔检测的应用包括候选药物的化合物筛选(初步筛选)、生物标记物验证(二次筛选)、siRNA 敲低效率、样品的前瞻性和/或回顾性分析、微阵列验证、预测毒理学以及检测易位和融合基因。
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)QuantiGene Plex 384 孔检测
适用于(设备)Invitrogen™Luminex™ FLEXMAP 3 D™ 仪器系统
产品线QuantiGene™
样品类型RNA
运输条件Blue Ice
检测方法Fluorescence
产品规格测定试剂盒(2块板)
产品类型QuantiGene Plex 384 孔检测试剂盒
数量2块板
种属Universal
Unit SizeEach
内容与储存

• 200 μL Proteinase K, -20°C
• 2.1 mL Blocking Reagent, 2°C to 8°C
• 31 mL Label Probe Solution, 2°C to 8°C
• 31 mL Pre-Amplifier Solution, 2°C to 8°C
• 31 mL Amplifier Solution, 2°C to 8°C
• 31 mL SAPE Solution, 2°C to 8°C
• 24 mL Lysis Mixture, 15–30°C
• 6 mL Wash Buffer Component 1, 15–30°C
• 100 mL Wash Buffer Component 2, 15–30°C
• 120 mL10x SAPE Wash Buffer Concentrate, 15–30°C
• 2 Hybridization plates (Day 1) 384-well, 15–30°C
• 4 Manual Pressure seals (Day 1), 15–30°C
• 4 Heat seals (Day 1), 15–30°C
• 2 Magnetic Separation plates (Day 2), 15–30°C
• 10 Plates seals (Day 2), 15–30°C

Note: Prepared 1x Wash Buffers can be stored for 1 week protected from light at 4°C.

常见问题解答 (FAQ)

Can samples in TRIzol solution be run in the QuantiGene Plex assay?

No, samples in TRIzol solution cannot be run in the QuantiGene Plex assay. However, RNA purified from these samples can be run in the QuantiGene Plex assay.

Can I use the QuantiGene Plex assay with bacteria?

Although the kits were not originally validated for bacteria, we have guidelines for preparing bacterial samples for QuantiGene Plex assays: http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf

Do you offer QuantiGene Plex assay custom services where I can send my samples for you to process?

Yes, please reach out to your Technical Sales Specialist for more information or fill out this form: https://www.thermofisher.com/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html

Can I detect genomic DNA using the QuantiGene Plex assay?

Under the hybridization conditions that are used for the QuantiGene Plex assay, genomic DNA remains double-stranded. Therefore, it is not possible for the probes to bind to DNA. The signal from the probe sets comes only from RNA.

Can I use other lysis buffers with QuantiGene Plex assay samples?

We only recommend the use of QuantiGene Lysis Mixture for preparing cell and blood lysates. The Lysis Mixture has been optimized to provide stability of RNA in the lysates. In addition, QuantiGene Lysis Mixture provides the appropriate buffer conditions for the overnight hybridization.