DTT(二硫苏糖醇)
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DTT(二硫苏糖醇)
Thermo Scientific™

DTT(二硫苏糖醇)

Thermo Scientific DTT(DL-二硫苏糖醇;Clelands 试剂)用于稳定含有游离巯基的酶和其他蛋白质。已证明其可恢复这些基团因体外氧化而丧失的活性。DTT 可定量还原二硫键,确保单硫醇处于还原状态(见参考文献了解更多信息
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货号数量
R08615 g
R086225 g
货号 R0861
价格(CNY)
647.00
飞享价
Ends: 31-Dec-2025
886.00
共减 239.00 (27%)
Each
添加至购物车
数量:
5 g
请求批量或定制报价
价格(CNY)
647.00
飞享价
Ends: 31-Dec-2025
886.00
共减 239.00 (27%)
Each
添加至购物车
Thermo Scientific DTT(DL-二硫苏糖醇;Clelands 试剂)用于稳定含有游离巯基的酶和其他蛋白质。已证明其可恢复这些基团因体外氧化而丧失的活性。DTT 可定量还原二硫键,确保单硫醇处于还原状态(参考文献 1)。

在最终 0.1 M 浓度下,DTT 也广泛用于破坏 SDS-聚丙烯酰胺凝胶电泳中的蛋白质二硫键。

产品优势

•不含内切或外切脱氧核糖核酸酶、核糖核酸酶和磷酸酶。

应用

•酶稳定
•酶促反应
•蛋白质电泳


• DTT 在溶液中不稳定。只能使用新鲜配置的DTT溶液。将 1.55 g DTT 粉末溶解在 10 mL 去离子水(如无核酸酶水,#R0581)中即可制备 1 M DTT 溶液。
仅供科研使用。不可用于诊断程序。
规格
产品类型DTT(二硫苏糖醇)
数量5 g
最大浓度0.1 M
Unit SizeEach

常见问题解答 (FAQ)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.