How long does it take to dissolve a Thermo Scientific TopVision Agarose Tablet?
Add the tablet into the determined amount of buffer, presoak for two minutes, and proceed the same way as you are used to with the traditional agarose powder. Using tablets saves 15-20 minutes hands-on-time.
At what voltage should I run an agarose gel prepared from TopVision Agarose Tablets?
The electrophoresis conditions do not depend on the form of delivery of agarose. Whether it is loose powder or tablets, the material has the same physicochemical conditions. In general, 4-10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) are recommended when running agarose gels under normal horizontal electrophoretic conditions.
Do TopVision Agarose Tablets perform the same compared to traditional agarose powder products?
Yes, the performance is the same, the convenience is much greater.The critical characteristics of agaroses (melting/gelling temperature, gel strength, electroendosmosis (EEO)) impacting the performance are the same.
What is the advantage of using agarose tablets rather than loose powder agarose?
A tablet is a more convenient format, eliminating the messy and time-consuming weighing step.