CellEvent™ Caspase-3/7 Green ReadyProbes™ 试剂
CellEvent™ Caspase-3/7 Green ReadyProbes™ 试剂
引用和文献 (29)
Invitrogen™

CellEvent™ Caspase-3/7 Green ReadyProbes™ 试剂

CellEvent™ 半胱天冬酶-3/7 Green ReadyProbes™ 试剂是活化半胱天冬酶-3/7 的荧光指示剂，且无需洗涤，适用于活细胞和固定细胞应用。半胱天冬酶-3 的活化是细胞凋亡的早期指标，并且 CellEvent™ 半胱天冬酶-3/7了解更多信息
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货号数量
R371111.75 mL
货号 R37111
价格（CNY)
2,917.00
Online Exclusive
Ends: 31-Dec-2026
4,051.00
共减 1,134.00 (28%)
Each
添加至购物车
数量:
1.75 mL
价格（CNY)
2,917.00
Online Exclusive
Ends: 31-Dec-2026
4,051.00
共减 1,134.00 (28%)
Each
添加至购物车
CellEvent™ 半胱天冬酶-3/7 Green ReadyProbes™ 试剂是活化半胱天冬酶-3/7 的荧光指示剂，且无需洗涤，适用于活细胞和固定细胞应用。半胱天冬酶-3 的活化是细胞凋亡的早期指标，并且 CellEvent™ 半胱天冬酶-3/7 Green 试剂可快速且灵敏地检测凋亡细胞。

•简单、快速的无需洗涤方案有助于保存易碎的凋亡细胞
• 同时兼容活细胞荧光成像法及甲醛固定法
• 即用型液体制剂，盛装于便利的滴瓶中—无需稀释、称量或量取
• 在室温下具有稳定性—使您的范围或细胞培养区域保持便利。

请参见其他用于细胞染色的 ReadyProbes™ 试剂
了解更多有关细胞凋亡的其他测定的信息

细胞成像应用
CellEvent™ 半胱天冬酶-3/7 Green 试剂是一种与核酸结合染料偶联的四氨基酸肽 (DEVD)，其未与 DNA 结合时不发出荧光。CellEvent™ 半胱天冬酶-3/7 Green 试剂本质上不发出荧光，因为 DEVD 多肽可抑制染料与 DNA 的结合。凋亡细胞中半胱天冬酶-3/7 活化后，DEVD 肽被切割，游离的染料可与 DNA 结合，从而产生明亮的绿色荧光。当染料与 DNA 结合时，在 530 nm 处有荧光发射，可以使用标准 FITC 滤光片组观察该荧光发射情况。

使用建议
• 大多数情况下，2 滴/mL 和 30 分钟的孵育时间足以对凋亡细胞进行明亮的细胞核染色；然而，对于某些细胞类型、条件和应用可能需要优化。在此种情况下，只需简单地加入更多或更少的液滴，直至获得较佳染色强度。
•如有需要，将 CellEvent™ 半胱天冬酶-3/7 Green ReadyProbes™ 试剂与红色或远红外细胞核染色剂结合，进行总细胞计数
• CellEvent™ 半胱天冬酶-3/7 Green 染料的最大激发波长为 502 nm，最大发射波长为 530 nm。通过标准 GFP 和 FITC 滤光片检测。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
适用细胞哺乳动物细胞
激发/发射502/530
适用于（应用）细胞成像
适用于（设备）共聚焦显微镜、Floid™ 细胞成像系统、荧光显微镜、流式细胞仪、Floid™ 细胞成像系统
标签类型其他标记或染料
产品线CellEvent，ReadyProbes
产品类型半胱天冬酶 3/7 试剂
数量1.75 mL
检测方法荧光
产品规格滴瓶
Unit SizeEach
内容与储存
1 × 1.75 mL 滴瓶

在 ≤ 25°C 下储存。

常见问题解答 (FAQ)

我能固定我的CellEvent Caspase 3/7标记的细胞么？

可以，在3.7%甲醛中固定15分钟后，CellEvent信号将会保留。 

我希望在微孔板实验中测定细胞增殖和细胞凋亡。您有推荐的产品么？

可以在同种样品中将PrestoBlue试剂（用于细胞增殖）和CellEvent Caspase 3/7 Green 检测试剂（用于细胞凋亡检测）结合。

流式细胞术的优势有哪些？

•可测定来自单个细胞的数据。
•可从大量细胞中获得数据，产生细胞群的丰富统计学分析结果。
•由于可测定单个细胞，能够揭示种群的异质性。
•支持多重检测，可鉴定小型亚群。
•可以快速分析数以千计的细胞。
•非常适合血液样本和其他悬浮细胞。
•数据获取后，可以多次重复分析。
•流式细胞仪文件（FCS）可以归档。

我能在流式细胞仪上进行哪些应用？

可进行多种应用，包括免疫分型、细胞周期分析、凋亡检测（如膜联蛋白V染色检测实验）、CellEvent Caspase-3/7检测、TUNEL检测、细胞活性检测、增殖检测（如CellTrace 检测和Click-iT EdU检测）、MitoProbe检测法测定线粒体电势、利用计数微球进行细胞计数。

I want to use your CellEvent Caspase-3/7 Green detection reagent for studying apoptosis in live cells, but for 96-well microplate instead of microscopy. Will it work? Can I multiplex it with a cell vitality dye?

Yes, on both accounts. We have demonstrated that it works for microplates, and we have multiplexed the dye with PrestoBlue Cell Viability Reagent. Some microplates may be less sensitive than microscopic analysis, so sensitivity may not be the same for the CellEvent reagent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase-3/7 Green ReadyProbes™ 试剂 FAQs

引用和文献 (29)

引用和文献
Abstract
SHOX triggers the lysosomal pathway of apoptosis via oxidative stress.
Authors:Hristov G, Marttila T, Durand C, Niesler B, Rappold GA, Marchini A,
Journal:
PubMed ID:24186869
The SHOX gene encodes for a transcription factor important for normal bone development. Mutations in the gene are associated with idiopathic short stature and are responsible for the growth failure and skeletal defects found in the majority of patients with Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia. SHOX is expressed ... More
Disruption of mitochondrial quality control genes promotes caspase-resistant cell survival following apoptotic stimuli.
Authors:
Journal:J Biol Chem
PubMed ID:35304098
Design and synthesis of novel 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4-thiadiazol- 2-yl)urea derivatives with potent anti-CML activity throughout PI3K/AKT signaling pathway.
Authors:Li W, Chu J, Fan T, Zhang W, Yao M, Ning Z, Wang M, Sun J, Zhao X, Wen A
Journal:Bioorg Med Chem Lett
PubMed ID:31097376
'In this investigation, a series of 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4- thiadiazol-2-yl)urea receptor tyrosine kinase inhibitors were synthesized by a simple and efficient structure-based design. Structure-activity relationship (SAR) analysis of these compounds based on cellular assays led to the discovery of a number of compounds that showed potent activity against human chronic myeloid leukemia ... More
SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial ?? in neuroblastoma cells.
Authors:Tanpure S, Boyineini J, Gnanamony M, Antony R, Fernández KS, Libes J, Lin J, Pinson D, Joseph PA, Gondi CS
Journal:Oncol Lett
PubMed ID:28599461
'Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27)' ... More
The Development of a Nano-based Approach to Alleviate Cisplatin-Induced Ototoxicity.
Authors:Kayyali MN, Ramsey AJ, Higbee-Dempsey EM, Yan L, O'Malley BW, Tsourkas A, Li D
Journal:J Assoc Res Otolaryngol
PubMed ID:29349595
'Cisplatin-induced hearing loss is experienced by a high percentage of patients with squamous cell carcinoma undergoing cisplatin chemotherapy. A novel nano-construct capable of sequestering extracellular cisplatin was developed to combat this problem. The nano-construct consisted of superparamagnetic iron oxide nanoparticles (SPIONs) entrapped within polymeric micelles, which were formed from a ... More
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